BIBLIOGRAPHY

Erspamer, Vittorio et al., 1993, Pharmacological Studies of 'Sapo' from the Frog Phyllomedusa bicolor Skin: A Drug Used by the Peruvian Matses Indians in Shamanic Hunting Practices, Toxicon, 31:1099-1111. [Ethnographic overview of the use of "hallucinogenic" frogs by Matses (largely from Gorman 1990) with proposed mechanism of action./KG] 

Erspamer, V. et al. "The Opioid Peptides of the Amphibian Skin", International Journal of Developmental Neuroscience 10: 3-30, 1992

Daly, J.W. et al. "Frog Secretions and Hunting Magic in the Upper Amazon: Identification of a Peptide that Interacts with an Adenosine Receptor", Proceedings of the National Academy of Sciences 89: 10960-10963, 1992.

Mignogna, G.; Severini, C.; Simmaca, M.; Negri, L.; Fal- 
conieri Erspamer, G.; Kreil, G.; Barra, D. Identification and 
characterization of two dermorphins from skin extracts of the 
Amazonian frog Phyllomedusa bicolor. FEBS Lett. 302:151 
154; 1992. 

Negri, L.; Falconieri Erspamer, G.; Severini, C.; Potenza, 
R. L.; Melchiori, P.; Erspamer, V. Dermorphin-related pep- 
tides from the skin of Phyllomedusa bicolor and their ami- 
dated analogs activate two opioid subtypes that modulate 
antinociceptine and catalepsy in the rat. Proc. Natl. Acad. Sci. 
USA. 89:72037207; 1992. 

Richter, K.; Egger, R.; Negri, L.; Corsi, R.; Severini, C.; Kreil, 
G. cDNAs encoding [D-Ala2]deltorphin precursors from skin
of Phyllomedusa bicolor also contain genetic information for
the dermorphin related opioid peptides. Proc. Natl. Acad. Sci.
USA. 87:48364839; 1990.

A Erspamer, P Melchiorry, G Falconieri-Erspamer, L Negri, R Corsi, C Severini, D Barra, M Simmaco, G Kreil: Deltorphins: a family of naturally occuring peptides with high affinity and selectivity for delta opioid binding sites.
Proc Ntl Acad Sci USA 1989 86: 5188-5192

 

ABSTRACTS:

Search History
*  #2 (phyllomedusa bicolor) in TI (7 records)
   #1 (erspamer- v.*) in AU (0 records)


Record 1 of 7 in BIOSIS Previews 2001/01-2001/03: 1-13

TI:  Peptide secretion in the cutaneous glands of South American tree frog Phyllomedusa bicolor: An ultrastructural study.
AU:  Lacombe-Claire {a}; Cifuentes-Diaz-Carmen; Dunia-Irene; Auber-Thomay-Michele; Nicolas-Pierre; Amiche-Mohamed
AD:  {a} Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, 2 Place Jussieu, F-75251, Paris Cedex 05: lacombe@ijm.jussieu.fr, France
SO:  European-Journal-of-Cell-Biology. [print] September, 2000; 79 (9): 631-641.
PY:  2000
DT:  Article-
IS:  0171-9335
LA:  English
LS:  English
AB:  The development of the dermal glands of the arboreal frog Phyllomedusa bicolor was investigated by immunocytochemistry and electron microscopy. The 3 types of glands (mucous, lipid and serous) differed in size and secretory activity. The mucous and serous glands were apparent in the tadpole skin, whereas the lipid glands developed later in ontogenesis. The peptide antibiotics dermaseptins and the D-amino acid-containing peptide opioids dermorphins and deltorphins are abundant in the skin secretions of P. bicolor. Although these peptides differ in their structure and activity they are derived from precursors that have very similar preproregions. We used an antibody to the common preproregion of preprodermaseptins and preprodeltorphins and immunofluorescence analysis to show that only the serous glands are specifically involved in the biosynthesis and secretion of dermaseptins and deltorphins. Scanning and transmission electron microscopy revealed that the serous glands of !
P. bicolor have morphological features, especially the secretory granules, which differ from those of the glands in Xenopus laevis skin.
AI:  Y
MC:  Integumentary-System (Chemical-Coordination-and-Homeostasis)
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
OR:  Phyllomedusa-bicolor [South-American-tree-frog] (Salientia-): tadpole-; Xenopus-laevis (Salientia-)
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
PS:  lipid-glands: integumentary-system; mucous-glands: integumentary-system; serous-glands: integumentary-system; skin-: integumentary-system
CB:  deltorphins-: biosynthesis-, secretion-; dermaseptins-: biosynthesis-, secretion-; dermorphins-: secretion-
RN:  182441-20-9: DELTORPHINS; 136212-91-4: DERMASEPTINS; 77614-16-5: DERMORPHINS
MI:  ontogenesis-
AN:  200100013443
UD:  20001219


Record 2 of 7 in Biosis Previews 1997/01-1997/06

TI:  Progressive ulcerative dermatitis in a captive, wild-caught South American giant tree frog (Phyllomedusa bicolor) with microsporidial septicemia.
AU:  Graczyk-Thaddeus-K {a}; Cranfield-Michael-R; Bicknese-Elizabeth-J; Wisnieski-Anthony-P
AD:  {a} Dep. Molecular Microbiol. Immunol., Sch. Hygiene Public Health, Johns Hopkins Univ., 615 North Wolfe St., Baltimore, MD 21205, USA
SO:  Journal-of-Zoo-and-Wildlife-Medicine. 1996; 27 (4) 522-527.
PY:  1996
DT:  Research-Article
IS:  1042-7260
LA:  English
AB:  One of 14 captive, wild-caught South American giant tree frogs (Phyllomedusa bicolor) displayed progressive ulcerative erythematous dermatitis on the dorsal skin lateral to the vertebral column and on the left hind leg. It had septicemia of unknown etiology. Tissue imprints revealed a mass of microsporidial spores. Red blood cells were parasitized by gamonts of Hepatozoon sp. (Apicomplexa: Hepatozooidae) or meronts of Babesiosoma stableri (Apicomplexa: Dactylostomatidae) or had inclusions and single or multiple cytoplasm lesions caused by frog erythrocytic virus. Monocytes had lesions in the nuclei and cytoplasm caused by frog leucocytic virus. Intramuscular injections with 6.25 mg of chloramphenicol sodium succinate for 18 days and topically administration of oxytetracycline HCl with polymyxin B sulfate for 21 days led to healing of the lesions. The total prevalence of infection for 14 P. bicolor was 50% for B. stableri and Hepatozoon sp., 36% for frog erythrocytic virus!
, and 21% for frog leucocytic virus.
AI:  Y
MC:  Blood-and-Lymphatics (Transport-and-Circulation); Cardiovascular-System (Transport-and-Circulation); Infection-; Integumentary-System (Chemical-Coordination-and-Homeostasis); Parasitology-; Pathology-; Veterinary-Medicine (Medical-Sciences)
ST:  Animal-Viruses-General: Viruses-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-; Sporozoa-: Invertebrata-, Protozoa-, Animalia-
OR:  animal-viruses (Animal-Viruses-General); Babesiosoma-stableri (Sporozoa-); Phyllomedusa-bicolor (Salientia-)
TN:  amphibians-; animals-; chordates-; invertebrates-; microorganisms-; nonhuman-vertebrates; protozoans-; vertebrates-; viruses-
CB:  CHLORAMPHENICOL-SODIUM-SUCCINATE; OXYTETRACYCLINE-HYDROCHLORIDE
RN:  982-57-0: CHLORAMPHENICOL SODIUM SUCCINATE; 2058-46-0: OXYTETRACYCLINE HYDROCHLORIDE
MI:  ANTIINFECTIVE-DRUG; ANTIINFLAMMATORY-DRUG; BLOOD-AND-LYMPHATICS; CHLORAMPHENICOL-SODIUM-SUCCINATE; DERMATOLOGICAL-DRUG; FROG-ERYTHROCYTIC-VIRUS; FROG-LEUKOCYTIC-VIRUS; HOST-; INTEGUMENTARY-SYSTEM; INTEGUMENTARY-SYSTEM-DISEASE; LEFT-HIND-LEG; MICROSPORIDIAL-SEPTICEMIA; OXYTETRACYCLINE-HYDROCHLORIDE; PARASITE-; PARASITIC-DISEASE; PARASITOLOGY-; PATHOGEN-; PROGRESSIVE-ULCERATIVE-ERYTHEMATOUS-DERMATITIS; RED-BLOOD-CELLS; SOUTH-AMERICAN-GIANT-TREE-FROG; SPORES-; VASCULAR-DISEASE; VETERINARY-MEDICINE
AN:  199799465739

Record 3 of 7 in Biosis Previews 1997/01-1997/06

TI:  Daily movements of male Phyllomedusa bicolor in Brazil.
AU:  Neckel-De-Oliveira-Selvino
AD:  Dep. de Ecol., Inst. Nacional de Pesquisas da Amazonia-INPA, CP 478, 69011-970 Manaus, Amazonas, Brazil
SO:  Herpetological-Review. 1996; 27 (4) 180-181.
PY:  1996
DT:  Research-Article
IS:  0018-084X
LA:  English
MC:  Behavior-; Physiology-; Systematics-and-Taxonomy
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
OR:  Phyllomedusa-bicolor (Salientia-)
TN:  amphibians-; animals-; chordates-; nonhuman-vertebrates; vertebrates-
GE:  Brazil- (South-America, Neotropical-region); South-America (Neotropical-region)
MI:  BEHAVIOR-; CALLING-SITE; DAILY-MOVEMENT; MALE-
AN:  199799358962

Record 4 of 7 in Biosis Previews 1993/07-1993/12

TI:  Pharmacological studies of "sapo" from the frog Phyllomedusa bicolor skin: A drug used by the Peruvian Matses Indians in shamanic hunting practices.
AU:  Erspamer-Vittorio {a}; Erspamer-Giuliana-Falconieri; Severini-Cinzia; Potenza-Rosa-Luisa {a}; Barra-Donatella; Mignogna-Giuseppina; Bianchi-Antonio
AD:  {a} Inst. Med. Pharmacol. III, Univ. Rome 'La Sapienza', Citta Univ., 00185 Rome, Italy
SO:  Toxicon-. 1993; 31 (9) 1099-1111.
PY:  1993
DT:  Article-
IS:  0041-0101
LA:  English
AB:  V. ERSPAMER, G. FALCONIERI ERSPAMER, C. SEVERINI, R. L. POTENZA, D. BARRA, G. MIGNOGNA and A. BIANCHI. Pharmacological studies of 'sapo' from the frog Phyllomedusa bicolor skin: a drug used by the Peruvian Matses Indians in shamanic hunting practices. Toxicon 31, 1099-1111, 1993. -The dried skin secretion from Phyllomedusa bicolor, 'sapo', is used by the Matses Indians of the Northern Peru, in shamanic rites mainly designed to improve luck in hunting. When rubbed into burned, exposed areas of the skin, the drug causes the prompt appearance of violent peripheral gastrointestinal and cardiovascular effects soon followed by remarkable central effects (increase in physical strength, heightening of senses, resistance to hunger and thirst, exalted capacity to face stress situations). All the peripheral and most of the central effects of 'sapo' can be ascribed to the exceptionally high content of the drug (up to 7% of its weight) in potently active peptides, easily absorbed thro!
ugh the burned, inflamed areas of the skin. The concentration in 'sapo' of the single peptides (phyllocaerulein, phyllomedusin, phyllokinin, dermorphins and deltorphins) has been determined by bioassay, and peptide contents were correlated with the different symptoms of the 'sapo' intoxication.
AI:  Y
MC:  Anthropology-; Behavior-; Neurology- (Human-Medicine, Medical-Sciences); Pharmacology-; Toxicology-
ST:  Hominidae-: Primates-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
OR:  Hominidae- (Hominidae-); Salientia- (Salientia-)
TN:  amphibians-; animals-; chordates-; humans-; mammals-; nonhuman-vertebrates; primates-; vertebrates-
CB:  PHYLLOCAERULEIN-; PHYLLOMEDUSIN-; PHYLLOKININ-; DERMORPHINS-; DELTORPHINS-
RN:  22415-80-1: PHYLLOCAERULEIN; 26145-48-2: PHYLLOMEDUSIN; 7265-10-3: PHYLLOKININ; 77614-16-5: DERMORPHINS; 119975-64-3: DELTORPHINS
MI:  DIAGNOSTIC-METHOD; ERYTHEMA-; IMMUNOGLOBULIN-E-MEDIATED-DRUG-ALLERGY; PRURITUS-
AN:  199396142926

Record 5 of 7 in BIOSIS Previews 1990 Part 2

TI:  COMPLEMENTARY DNAS ENCODING D-ALA-2 DELTORPHIN PRECURSORS FROM SKIN OF PHYLLOMEDUSA-BICOLOR ALSO CONTAIN GENETIC INFORMATION FOR THREE DERMORPHIN-RELATED OPIOID PEPTIDES.
AU:  RICHTER-K {a}; EGGER-R; NEGRI-L; CORSI-R; SEVERINI-C; KREIL-G
AD:  {a} INST MOL BIOL, AUSTRIAN ACAD OF SCI BILLROTHSTRASSE 11, A-5020 SALZBURG, AUST
SO:  Proceedings-of-the-National-Academy-of-Sciences-of-the-United-States-of-America. 1990; 87 (12): 4836-4839.
PY:  1990
DT:  Article-
IS:  0027-8424
LA:  ENGLISH
AB:  We present the structure of four precursors for [D-Ala2]deltorphins I and II as deduced from cDNAs cloned from skin of the frog Phyllomedusa bicolor. These contain the genetic information for one copy of [D-Ala2]deltorphin II and zero, one, or three copies of [D-Ala2] deltorphin I. In each case, the D-alanine of the end product is encoded by a normal GCG codon for L-alanine. In addition, the existence of three peptides related to dermorphin was predicted from the amino acid sequence of the precursors. These peptides were synthesized with a D-alanine in position 2 and their pharmacological properties were tested. Two of them, [Lys7]dermorphin-OH and [Trp4,Asn7]dermorphin-OH, were found to have roughly the same affinity and selectivity for .mu.-type opioid receptors as dermorphin.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  FROG
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC03506 (Genetics-and-Cytogenetics-Animal)
CC10062 (Biochemical-Studies-Nucleic-Acids-Purines-and-Pyrimidines)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
MNCC:  CC10052 (Biochemical-Methods-Nucleic-Acids-Purines-and-Pyrimidines)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC18501 (Integumentary-System-General-Methods)
BC:  BC85306 Salientia
AN:  199090063473
UD:  1990

Record 6 of 7 in BIOSIS Previews 1992 Part 2

TI:  DERMORPHIN-RELATED PEPTIDES FROM THE SKIN OF PHYLLOMEDUSA-BICOLOR AND THEIR AMIDATED ANALOGS ACTIVATE TWO MU OPIOID RECEPTOR SUBTYPES THAT MODULATE ANTINOCICEPTION AND CATALEPSY IN THE RAT.
AU:  NEGRI-L {a}; FALCONIERI-ERSPAMER-G; SEVERINI-C; POTENZA-R-L; MELCHIORRI-P; ERSPAMER-V
AD:  {a} INST PHARMACOL, UNIV LA SAPIENZA, 00185 ROME, ITALY
SO:  Proceedings-of-the-National-Academy-of-Sciences-of-the-United-States-of-America. 1992; 89 (15): 7203-7207.
PY:  1992
DT:  Article-
IS:  0027-8424
LA:  ENGLISH
AB:  Three naturally occurring dermorphin-like peptides from the skin of the frog Phyllomedusa bicolor, the related carboxyl-terminal amides, and some substituted analogs were synthesized, their binding profiles to opioid receptors were determined, and their biological activities were studied in isolated organ preparations and intact animals. The opioid binding profile revealed a very high selectivity of these peptides for .mu. sites and suggested the existence of two receptors subtypes, of high and low affinity. The peptides tested acted as potent .mu. opioid agonists on isolated organ preparations. They were several times more active in inhibiting electrically evoked contractions in guinea pig ileum than in mouse vas deferens. When injected into the lateral brain ventricle or peritoneum of rats, the high-affinity-site-preferring ligand, [Lys7-NH2]dermorphin, behaved as a potent analgesic agent. By contrast, the low-affinity-site-preferring ligand, [Trp4,Asn7-NH2]dermorphin, !
produced a weak antinociception but an intense catalepsy.
AI:  Y
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  GUINEA-PIG MOUSE 7 LYSINAMIDE DERMORPHIN 4 TRYPTOPHAN-7-ASPARAGINAMIDE DERMORPHIN ANALGESIC-DRUG HIGH-AFFINITY RECEPTOR LOW-AFFINITY RECEPTOR
MJCC:  CC22024 (Pharmacology-Neuropharmacology)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC10508 (Biophysics-Membrane-Phenomena)
CC17020 (Endocrine-System-Neuroendocrinology)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC22016 (Pharmacology-Endocrine-System)
BC:  BC86300 Caviidae; BC86375 Muridae
AN:  199294124455
UD:  1992

Record 7 of 7 in BIOSIS Previews 1992 Part 2

TI:  IDENTIFICATION AND CHARACTERIZATION OF TWO DERMORPHINS FROM SKIN EXTRACTS OF THE AMAZONIAN FROG PHYLLOMEDUSA-BICOLOR.
AU:  MIGNOGNA-G {a}; SEVERINI-C; SIMMACO-M; NEGRI-L; ERSPAMER-G-F; KREIL-G; BARRA-D
AD:  {a} DIP SCI BIOCHIM, UNIV LA SAPIENZA, PIAZZALE ALDO MORO 5, 00185 ROMA, ITALY
SO:  Febs-Letters. 1992; 302 (2): 151-154.
PY:  1992
DT:  Article-
IS:  0014-5793
LA:  ENGLISH
AB:  Skin extracts of South American hylid frogs of the subfamily Phyllomedusinae contain dermorphins and deltorphins, opioid heptapeptides highly selective for either .mu. or .delta. receptors. In all these peptides, a D-amino acid is present in the second position. The structure of the precursors for Ala-deltorphins was recently deduced from cloned cDNAs derived from skin of Phyllomedusa bicolor (Richter et al. (1990) Proc. Natl. Acad. Sci. USA 87, 4836-4839). From the amino acid sequence of these precursors, the existence of three peptides related to dermorphin could be predicted. From methanol extracts of skin of P. bicolor we have isolated two of these peptides, [Lys7]dermorphin-OH and [Trp4,Asn7]dermorphin-OH. The biological activity of these new dermorphins and their amidated counterparts is presented.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PHYLLOMEDUSINAE SUBFAMILY COMPLEMENTARY DNA 7 LYSINE DERMORPHIN 4 TRYPTOPHAN-7-ASPARAGINE DERMORPHIN MOLECULAR SEQUENCE DATA AMINO ACID SEQUENCE
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC17020 (Endocrine-System-Neuroendocrinology)
MNCC:  CC10062 (Biochemical-Studies-Nucleic-Acids-Purines-and-Pyrimidines)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
BC:  BC85306 Salientia
AN:  199294040474
UD:  1992

 

**********************

Search History
*  #2 'Erspamer-V' in AU (37 records)
   #1 (erspamer-v*) in AU (50 records)


Record 1 of 37 in BIOSIS Previews 2001/01-2001/03: 1-13

TI:  Effects of natural tachykinins on porcine lower urinary tract smooth muscle.
AU:  Bolle-P {a}; Severini-C; Falconieri-Erspamer-G; Tucci-P; Erspamer-V
AD:  {a} Department of Pharmacology and Physiology, University of Rome 'La Sapienza', Rome, Italy
SO:  Journal-of-Autonomic-Pharmacology. [print] June, 2000; 20 (3): 157-161.
PY:  2000
DT:  Article-
IS:  0144-1795
LA:  English
LS:  English
AB:  1 The aim of our study was to ascertain the possible differences and/or similarities in natural tachykinin activity in vitro on lower urinary tract of large-sized animals as compared with data obtained in laboratory animals. 2 Besides tachykinins normally present in mammals, namely substance P (SP), neurokinin A (NKA) and neurokinin B (NKB), we tested non-mammalian tachykinins, such as eledoisin (ELED), physalaemin (PHYS), kassinin (KASS) and PG-kassinin II (PG-KASS II). 3 NKA, KASS and ELED were found to be the most potent peptides in contracting detrusor strips from porcine bladder. In particular, NKA showed a pD2 of 7.14, whereas KASS and ELED showed pD2 values of 7.20 and 7.22, respectively. The activity of NKB and PG-KASS II corresponded to 72.4 and 55.0% respectively of that of NKA. SP and PHYS activity corresponded to only 2% of that of NKA. 4 NKA (pD2 7.92) was the most active peptide in contracting bladder neck tissues as well. ELED and KASS were found to have lo!
wer, similar pD2 values (7.62 and 7.70, respectively), whereas NKB and PG-KASS II were much less active (pD2 7.12 and 6.74, respectively). Moreover, SP and PHYS showed an activity range lower than 2% of that of NKA. 5 The reported results confirm that, on pig vesical neck and detrusor, NK1 receptors represent a minority as compared with NK2 and NK3 receptors. By contrast, the presence of NK2 receptors is demonstrated by a greater potency of NKA. The presence of NK3 receptors both on detrusor and neck is evidenced by NKB activity and by results achieved with PG-KASS II.
AI:  Y
MC:  Biochemistry-and-Molecular-Biophysics; Urinary-System (Chemical-Coordination-and-Homeostasis)
ST:  Suidae-: Artiodactyla-, Mammalia-, Vertebrata-, Chordata-, Animalia-
OR:  porcine- (Suidae-)
TN:  Animals-; Artiodactyls-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Vertebrates-
PS:  bladder-: excretory-system; urinary-tract-smooth-muscle: excretory-system, muscular-system
CB:  PG-kassinin-II: activity-, tachykinin-; eledoisin-: activity-, tachykinin-; kassinin-: activity-, tachykinin-; neurokinin-A: activity-, tachykinin-; neurokinin-B: activity-, tachykinin-; neurokinin-1-receptors; neurokinin-2-receptors; neurokinin-3-receptors; physalaemin-: activity-, tachykinin-; substance-P: activity-, tachykinin-
RN:  69-25-0: ELEDOISIN; 63968-82-1: KASSININ; 86933-74-6: NEUROKININ A; 102577-23-1: NEUROKININ B; 2507-24-6: PHYSALAEMIN; 33507-63-0: SUBSTANCE P
AN:  200100062221
UD:  20010123


Record 2 of 37 in Biosis Previews 1999/01-1999/06

TI:  Transmitter release and uptake evoked by the amphibian skin alkaloid, pumiliotoxin-B (PTX-B), in the electrically stimulated mouse vas deferens preparation (MVD).
AU:  Severini-C; Falconieri-Erspamer-G; Erspamer-V {a}
AD:  {a} Med. Pharmacol. III, Univ. Rome "La Sapienza', Citta Univ., Piazzale Aldo Moro 5, 00185 Rome, Italy
SO:  Journal-of-Autonomic-Pharmacology. Dec., 1998; 18 (6) 333-342.
PY:  1998
DT:  Article-
IS:  0144-1795
LA:  English
AB:  1. Upon electrical stimulation three transmitters are known to be released from the adrenergic nerve terminals of the isolated MVD preparation: two motor transmitters (noradrenaline (NA) and ATP) acting synergistically to provoke twitch contraction, and an inhibitory transmitter, the peptide NPY. 2. The frog alkaloid pumiliotoxin-B (PTX-B) displayed two opposite effects on the electrically stimulated MVD: at low concentrations (0.1-0.3 mum) it caused twitch depression, at higher concentrations (0.5-2 mum) there was a potent twitch stimulation. Transmitters and/or receptors involved in the depressive effect could not be clearly identified, although interference with NPY is possible. On the other hand, the potent twitch stimulation caused by PTX-B may be due to exaggerated release of the same transmitters (NA and ATP) involved in twitch stimulation produced by electrical stimulation. 3. Opening by PTX-B of the Na+ channels on the membrane of the adrenergic nerve terminals c!
auses activation of the amine pump facilitating re-uptake of not only endogenous NA but also of exogenous catecholamines.
AI:  Y
MC:  Nervous-System (Neural-Coordination); Pharmacology-
ST:  Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
OR:  frog- (Salientia-); mouse- (Muridae-)
TN:  Amphibians-; Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
PS:  vas-deferens: reproductive-system
CB:  catecholamine-; neuropeptide-Y; noradrenaline- [norepinephrine-]; pumiliotoxin-B: amphibian-skin-alkaloid, pharmacodynamics-; sodium-ion-channels; transmitter-: release-, uptake-; ATP-
RN:  51-41-2: NORADRENALINE; 51-41-2: NOREPINEPHRINE; 56-65-5Q: ATP; 42530-29-0Q: ATP; 94587-45-8Q: ATP; 111839-44-2Q: ATP; 67016-65-3: PUMILIOTOXIN B; 17341-25-2: SODIUM ION
AN:  199900105108
UD:  19990225

Record 3 of 37 in BIOSIS Previews 1985 Part 1

TI:  BOVINE MILK BOMBESIN PARTIAL PURIFICATION CHARACTERIZATION AND BIOACTIVITY.
AU:  LAZARUS-L-H {a}; GAUDINO-G; WILSON-W-E; ERSPAMER-V
AD:  {a} LBNT, NIEHS, NIH, PO BOX 12233, RESEARCH TRIANGLE PARK, 27709
SO:  Abstracts-of-Papers-American-Chemical-Society. 1984; 188: NO PAGINATION.
PY:  1984
DT:  Meeting-
IS:  0065-7727
MT:  188TH AMERICAN CHEMICAL SOCIETY MEETING, PHILADELPHIA, PA., USA, AUG. 26-31, 1984. ABSTR PAP AM CHEM SOC
LA:  ENGLISH
ST:  Bovidae-: Artiodactyla-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Artiodactyls-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Vertebrates-
MI:  ABSTRACT
MJCC:  CC17002 (Endocrine-System-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC16504 (Reproductive-System-Physiology-and-Biochemistry)
CC17020 (Endocrine-System-Neuroendocrinology)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
BC:  BC85715 Bovidae
AN:  198528087043
UD:  1985

Record 4 of 37 in BIOSIS Previews 1985 Part 1

TI:  HALF A CENTURY OF COMPARATIVE RESEARCH ON BIOGENIC AMINES AND ACTIVE PEPTIDES IN AMPHIBIAN SKIN AND MOLLUSCAN TISSUES.
AU:  ERSPAMER-V {a}
AD:  {a} INST MED PHARMACOLOGY, 1ST UNIV ROME, CITTA UNIVERSITARIA, 00100 ROMA, ITALY
SO:  Comparative-Biochemistry-and-Physiology-C-Pharmacology-Toxicology-and-Endocrinology. 1984; 79 (1): 1-8.
PY:  1984
DT:  Article-
IS:  0742-8413
LA:  ENGLISH
ST:  Cephalopoda-: Mollusca-, Invertebrata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Invertebrates-; Mollusks-; Nonhuman-Vertebrates; Vertebrates-
MI:  ELEDONE-MOSCHATA PHYSALAEMUS-BILIGONIGERUS DISCOGLOSSUS-PICTUS OCTOPUS-VULGARIS CHOLINE ESTER PHENYLALKYLAMINE IMIDAZOLEALKYLAMINE INDOLEALKYLAMINE CHROMATOGRAPHY
MJCC:  CC17002 (Endocrine-System-General)
CC10010 (Comparative-Biochemistry-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC64026 (Invertebrata-Comparative-and-Experimental-Morphology-Physiology-and-Pathology-Mollusca)
MNCC:  CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10504 (Biophysics-General-Biophysical-Techniques)
CC18501 (Integumentary-System-General-Methods)
BC:  BC61100 Cephalopoda; BC85306 Salientia
AN:  198528037554
UD:  1985

Record 5 of 37 in BIOSIS Previews 1985 Part 1

TI:  STIMULATION OF CROP SAC AND PITUITARY LACTOTROPHS AFTER INTRAVENTRICULAR ADMINISTRATION OF DERMORPHIN IN PIGEONS COLUMBA-LIVIA.
AU:  ROTIROTI-D {a}; GERMANA-G; NISTICO-G; MELCHIORRI-P; ERSPAMER-V
AD:  {a} INST PHARMACOL, FAC MED SURG, CATANZARO, OSPEDALE MADONNA DEI CIELI, 88100 CATANZARO, ITALY
SO:  General-and-Comparative-Endocrinology. 1984; 56 (1): 24-31.
PY:  1984
DT:  Article-
IS:  0016-6480
LA:  ENGLISH
AB:  The effects of dermorphin, a new potent opioid peptide, on structural and ultrastructural changes in the crop sac and in pituitary lactotropes were assessed in pigeons (Columba livia). A single administration of dermophin into the 3rd cerebral ventricle (1 .mu.g) and the same dose repeated for 3 consecutive days both produced maximal crop sac stimulation, as documented by the presence of milk-like material as well as by scanning and transmission electron microscopy of crop sac mucosa. Marked stimulatory effects were also observed in pituitary lactotropes, as shown by stimulation of Golgi apparatus, enlargement of rough endoplasmic reticulum, increase in mitochondria size, and the presence of lysosomes and electron-opaque granules. The stimulatory effects of dermorphin on the crop sac and lactotropes were completely prevented by naloxone, an antagonist at opiate receptors. Since dermorphin is a natural constituent of the avian brain, this peptide may represent the still-ob!
scure hypothalamic prolactin stimulatory factor.
AI:  Y
ST:  Columbiformes-: Aves-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Birds-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  AVIAN BRAIN CEREBRAL VENTRICLE HORMONE-DRUG NALOXONE AUTONOMIC-DRUG OPIATE RECEPTOR ANTAGONIST NEW OPIOID PEPTIDE HYPOTHALAMIC PROLACTIN STIMULATORY FACTOR PHARMACODYNAMICS
MJCC:  CC17014 (Endocrine-System-Pituitary)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC11108 (Anatomy-and-Histology-General-and-Comparative-Microscopic-and-Ultramicroscopic-Anatomy)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC22016 (Pharmacology-Endocrine-System)
CC22024 (Pharmacology-Neuropharmacology)
MNCC:  CC01056 (Microscopy-Techniques-Histology-and-Histochemistry)
CC01058 (Microscopy-Techniques-Electron-Microscopy)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC20501 (Nervous-System-General-Methods)
CC22014 (Pharmacology-Digestive-System)
CC22100 (Routes-of-Immunization-Infection-and-Therapy)
BC:  BC85524 Columbiformes
AN:  198579021041
UD:  1985

Record 6 of 37 in BIOSIS Previews 1985 Part 2

TI:  A POTENT FACTOR IN EXTRACTS OF THE SKIN OF THE AUSTRALIAN FROG PSEUDOPHRYNE-CORIACEA APPARENT FACILITATION OF TRANSMITTER RELEASE IN ISOLATED SMOOTH MUSCLE PREPARATIONS.
AU:  ERSPAMER-V {a}; ERSPAMER-G-F; MELCHIORRI-P; MAZZANTI-G
AD:  {a} INST MED PHARMACOL PHARMACOL PHARMACOGNOSY, 1ST UNIV ROME, CITTA UNIV, 00100 ROME, ITALY
SO:  Neuropharmacology-. 1985; 24 (8): 783-792.
PY:  1985
DT:  Article-
IS:  0028-3908
LA:  ENGLISH
AB:  Extracts of the skin of the Australian frog, P. coriacea, displayed potent stimulant effects on isolated smooth muscle preparations of intestine and similar effects on electrically-stimulated vas deferens preparations. These effects must be ascribed to an alkaloid, related in structure to the pumiliotoxins, a class of alkaloid compounds occurring in the skin of neotropical poison frogs. The extract has a pre-synaptic, neurogenic point of attack and it acts to facilitate the release of transmitters from nerve endings. Acetylcholine is the most important agent involved in the response to the extract by the intestinal muscle and noradrenaline [norepinephrine] in the response by vas deferens preparations. However, release of other aminergic or peptidergic transmitters may participate, positively or negatively, in the response.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PUMILIOTOXIN ACETYLCHOLINE NOREPINEPHRINE ELECTROPHYSIOLOGY NEUROGENIC ATTACK VAS DEFERENS INTESTINE
MJCC:  CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC16504 (Reproductive-System-Physiology-and-Biochemistry)
CC17020 (Endocrine-System-Neuroendocrinology)
CC18501 (Integumentary-System-General-Methods)
CC20501 (Nervous-System-General-Methods)
CC22501 (Toxicology-General-Methods-and-Experimental)
MNCC:  CC10060 (Biochemical-Studies-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10504 (Biophysics-General-Biophysical-Techniques)
CC10610 (External-Effects-Electric-Magnetic-and-Gravitational-Phenomena)
BC:  BC85306 Salientia
AN:  198580097742
UD:  1985

Record 7 of 37 in BIOSIS Previews 1985 Part 2

TI:  TRYPTOPHYLLINS FROM EXTRACTS OF PHYLLOMEDUSA-RHODEI SKIN NEW TETRAPEPTIDES PENTAPEPTIDES AND HEPTAPEPTIDES FURTHER STUDIES.
AU:  GOZZINI-L {a}; MONTECUCCHI-P-C; ERSPAMER-V; MELCHIORRI-P
AD:  {a} FARMITALIA CARLO ERBA SPA RICERCA AND SVILUPPO CHIMICO, VIA DEI GRACCHI 35, 20146 MILAN, ITALY
SO:  International-Journal-of-Peptide-and-Protein-Research. 1985; 25 (3): 323-329.
PY:  1985
DT:  Article-
IS:  0367-8377
LA:  ENGLISH
AB:  Tryptophyllins (TPH) are members of a new family of amphibian skin peptides containing in their structures a tryptophyl residue. The identification of tetra-, penta- and heptapeptides occurring in the 70% ethanol eluates from alkaline alumina columns of skin extracts of P. rhodei is described.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
MNCC:  CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10504 (Biophysics-General-Biophysical-Techniques)
CC18501 (Integumentary-System-General-Methods)
CC32600 (In-Vitro-Studies-Cellular-and-Subcellular)
BC:  BC85306 Salientia
AN:  198580013535
UD:  1985

Record 8 of 37 in BIOSIS Previews 1986 Part 1

TI:  PRIMARY STRUCTURE DETERMINATION OF A TRYPTOPHAN-CONTAINING TRIDECAPEPTIDE FROM PHYLLOMEDUSA-ROHDEI.
AU:  MONTECUCCHI-P-C {a}; GOZZINI-L; ERSPAMER-V
AD:  {a} FARMITALIA CARLO ERBA SPA, RICERCA AND SVILUPPO CHIMICO, VIA DEI GRACCHI 35, 20156 MILANO, ITALY
SO:  International-Journal-of-Peptide-and-Protein-Research. 1986; 27 (2): 175-182.
PY:  1986
DT:  Article-
IS:  0367-8377
LA:  ENGLISH
AB:  A new tryptophan-containing peptide has been identified in the methanol extracts of the skin of Phyllomedusa rohdei. The primary structure proposed is the following: <Glu-Glu-Lys-Pro-Tyr-Trp-Pro-Pro-Pro-Ile-Tyr-Pro-Met-OH.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MJCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC17020 (Endocrine-System-Neuroendocrinology)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC10504 (Biophysics-General-Biophysical-Techniques)
BC:  BC85306 Salientia
AN:  198681108376
UD:  1986

Record 9 of 37 in BIOSIS Previews 1986 Part 2

TI:  PEPTIDES OF THE AMINE PRECURSOR UPTAKE DECARBOXYLATION SYSTEM IN AMPHIBIAN SKINS.
AU:  DE-MAGISTRIS-L {a}; ANNIBALE-B; DELLE-FAVE-G; SALERA-M; PUOTI-M; GIORDANO-E; FORTE-A; ERSPAMER-V
AD:  {a} CATTEDRA DI GASTROENTEROLOGIA 1, CLINICA MEDICA 2, POLICLINICO UMBERTO 1, 00161 ROMA, ITALY
SO:  Peptides-New-York. 1985 (RECD. 1986); 6 (SUPPL. 3): 203-208.
PY:  1985 (RECD 1986)
DT:  Meeting-
IS:  0196-9781
MT:  FIRST INTERNATIONAL SYMPOSIUM ON NONMAMMALIAN PEPTIDES, ROME, ITALY, MAY 11-15, 1985. PEPTIDES (FAYETTEVILLE)
LA:  ENGLISH
ST:  Amphibia-: Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  CALCITONIN GASTRIN GASTROINTESTINAL PEPTIDE MOTILIN PANCREATIC POLYPEPTIDE VASOACTIVE INTESTINAL PEPTIDE
MJCC:  CC17002 (Endocrine-System-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17010 (Endocrine-System-Parathyroid)
CC17018 (Endocrine-System-Thyroid)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
BC:  BC85300 Amphibia-Unspecified
AN:  198631064912
UD:  1986

Record 10 of 37 in BIOSIS Previews 1986 Part 2

TI:  BOMBESIN EFFECTS ON HUMAN GASTROINTESTINAL FUNCTIONS.
AU:  DELLE-FAVE-G {a}; ANNIBALE-B; DE-MAGISTRIS-L; SEVERI-C; BRUZZONE-R; PUOTI-M; MELCHIORRI-P; TORSOLI-A; ERSPAMER-V
AD:  {a} CATTEDRA DI GASTROENTEROLOGIA, 1ST CLINICA MEDICA 2, POLICLINICO, I-00161 ROMA, ITALIA
SO:  Peptides-New-York. 1985 (RECD. 1986); 6 (SUPPL. 3): 113-116.
PY:  1985 (RECD 1986)
DT:  Meeting-
IS:  0196-9781
MT:  FIRST INTERNATIONAL SYMPOSIUM ON NONMAMMALIAN PEPTIDES, ROME, ITALY, MAY 11-15, 1985. PEPTIDES (FAYETTEVILLE)
LA:  ENGLISH
ST:  Hominidae-: Primates-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Humans-; Mammals-; Primates-; Vertebrates-
MI:  GASTRIC PANCREATIC EXOCRINE ENDOCRINE SECRETION
MJCC:  CC17002 (Endocrine-System-General)
CC10006 (Clinical-Biochemistry-General-Methods-and-Applications)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17008 (Endocrine-System-Pancreas)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
BC:  BC86215 Hominidae
AN:  198631064897
UD:  1986

Record 11 of 37 in BIOSIS Previews 1986 Part 2

TI:  EFFECTS OF SAUVAGINE ON BEHAVIORAL AROUSAL OF MICE.
AU:  PAVONE-F {a}; CASTELLANO-C; OLIVERIO-A; MELCHIORRI-P; ERSPAMER-V
AD:  {a} ISTITUTO DI PSICOBIOLOGIA E PSICOFARMACOLOGIA, VIA RENO 1 00198 ROME, ITALY
SO:  Peptides-New-York. 1985 (RECD. 1986); 6 (SUPPL. 3): 59-62.
PY:  1985 (RECD 1986)
DT:  Meeting-
IS:  0196-9781
MT:  FIRST INTERNATIONAL SYMPOSIUM ON NONMAMMALIAN PEPTIDES, ROME, ITALY, MAY 11-15, 1985. PEPTIDES (FAYETTEVILLE)
LA:  ENGLISH
ST:  Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  LOCOMOTOR ACTIVITY ENDOGENOUS OPIOID
MJCC:  CC07003 (Behavioral-Biology-Animal-Behavior)
CC17002 (Endocrine-System-General)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC21003 (Psychiatry-Psychophysiology)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
BC:  BC86375 Muridae
AN:  198631064888
UD:  1986

Record 12 of 37 in BIOSIS Previews 1986 Part 2

TI:  PHYLLOMEDUSA SKIN A HUGE FACTORY AND STORE-HOUSE OF A VARIETY OF ACTIVE PEPTIDES.
AU:  ERSPAMER-V {a}; MELCHIORRI-P; ERSPAMER-G-F; MONTECUCCHI-P-C; DE-CASTIGLIONE-R
AD:  {a} INST OF MED PHARMACOL III, UNIV OF ROME "LA SAPIENZA" CITTA UNIVERSITARIA, 00100 ROME, ITALY
SO:  Peptides-New-York. 1985 (RECD. 1986); 6 (SUPPL. 3): 7-12.
PY:  1985 (RECD 1986)
DT:  Meeting-
IS:  0196-9781
MT:  FIRST INTERNATIONAL SYMPOSIUM ON NONMAMMALIAN PEPTIDES, ROME, ITALY, MAY 11-15, 1985. PEPTIDES (FAYETTEVILLE)
LA:  ENGLISH
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PHYLLOCAERULEIN PHYLLOMEDUSIN PHYLLOKININ PHYLLOLITORIN SAUVAGINE DERMORPHIN
MJCC:  CC17002 (Endocrine-System-General)
CC10010 (Comparative-Biochemistry-General)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
BC:  BC85306 Salientia
AN:  198631064880
UD:  1986

Record 13 of 37 in BIOSIS Previews 1986 Part 2

TI:  A POTENT FACTOR IN EXTRACTS OF THE SKIN OF THE AUSTRALIAN FROG PSEUDOPHRYNE-CORIACEA III. POTENTIATION OF CONTRACTIONS ELICITED IN AVIAN AND MAMMALIAN ISOLATED SKELETAL MUSCLE PREPARATIONS BY DIRECT AND INDIRECT ELECTRICAL STIMULATION.
AU:  FALCONIERI-ERSPAMER-G {a}; ERSPAMER-V; MELCHIORRI-P
AD:  {a} INST PHARMACOL PHARMACOGNOSY, FIRST UNIV ROME, CITTA UNIV, 00100 ROME, ITALY
SO:  Neuropharmacology-. 1986; 25 (8): 807-814.
PY:  1986
DT:  Article-
IS:  0028-3908
LA:  ENGLISH
AB:  Extracts of the skin of the Australian frog Pseudophryne coriacea displayed a striking potentiating effect on contractions evoked in isolated skeletal muscle preparation of mammals (phrenic nerve diaphragm) and birds (chick biventer cervicis and semispinalis muscles) by indirect and direct electrical stimulation. There was both a conspicuous increase in the amplitude of the twitch, up to 10-fold, and a remarkable prolongation of the duration of the twitch. The effect was dose- and frequency-dependent. In the presence of the extract, fusion of twitches after tetanic stimulation occurred earlier. No tachyphylaxis upon repeated stimulation by the extract was observed and the response to large doses persisted, declining slowly, for hours. These effects must be ascribed to an alkaloid related in structure to pumiliotoxin B. Response to the extract of Pseudophryne coriacea by indirectly-stimulated preparations was potentiated by physostigmine and blocked by tubocurarine and  .a!
lpha.-bungarotoxin, demonstrating that in these preparations the extract acted pre-synaptically to facilitate the release of acetylcholine from motor nerve endings. However, the extract of Pseudophryne coriaceae displayed equally potent effects in directly stimulated preparations, insensitive to physostigmine and to blockers of nicotinic acetylcholine receptors, indicating a direct action on the skeletal muscle. It is suggested that, like pumiliotoxin B, the Pseudophryne, coriacea alkaloid may interfere in the regulation of calcium channels in both nerve and muscle fibres.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-; Galliformes-: Aves-, Vertebrata-, Chordata-, Animalia-; Cricetidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Birds-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  CALCIUM ACETYLCHOLINE PHARMACODYNAMICS
MJCC:  CC22022 (Pharmacology-Muscle-System)
CC17504 (Muscle-Physiology-and-Biochemistry)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC22024 (Pharmacology-Neuropharmacology)
MNCC:  CC10060 (Biochemical-Studies-General)
CC10069 (Biochemical-Studies-Minerals)
CC13010 (Metabolism-Minerals)
CC18501 (Integumentary-System-General-Methods)
BC:  BC85306 Salientia; BC85536 Galliformes; BC86310 Cricetidae; BC86375 Muridae
AN:  198682115045
UD:  1986

Record 14 of 37 in BIOSIS Previews 1986 Part 2

TI:  AN IMMUNOREACTIVE PEPTIDE IN MILK CONTAINS BOMBESIN-LIKE BIOACTIVITY.
AU:  LAZARUS-L-H {a}; GAUDINO-G; WILSON-W-E; ERSPAMER-V
AD:  {a} PEPTIDE NEUROCHEM GROUP, LAB BEHAV NEUROL TOXICOL, NATL INST ENVIRON HEALTH SCI, PO BOX 1233, RESEARCH TRIANGLE PARK, NC 27709, USA
SO:  Experientia-Basel. 1986; 42 (7): 822-823.
PY:  1986
DT:  Article-
IS:  0014-4754
LA:  ENGLISH
AB:  Parallel in vitro bioassays using rat uterus and guinea pig large intestine tissues specific for the bombesin family of peptides, demonstrated that the bombesin-like peptides present in bovine milk can produce at dose-related response similar to bombesin and litorin. The bioactivity of this type of milk peptide appeared to be approximately 20-50% as active as the amphibibian peptides. These data support the proposal that a bombesin immunoreactive peptide in milk contains bombesin bioactivity.
AI:  Y
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  RAT GUINEA-PIG LITORIN
MJCC:  CC17002 (Endocrine-System-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC13518 (Food-Technology-Dairy-Products)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC16504 (Reproductive-System-Physiology-and-Biochemistry)
CC34502 (Immunology-and-Immunochemistry-General-Methods)
MNCC:  CC13530 (Food-Technology-Evaluations-of-Physical-and-Chemical-Properties)
BC:  BC86300 Caviidae; BC86375 Muridae
AN:  198682081546
UD:  1986

Record 15 of 37 in BIOSIS Previews 1987 Part 1

TI:  SYNTHESIS AND BIOLOGICAL ACTIVITY OF THE SAUVAGINE FRAGMENT 18-40.
AU:  WENDLBERGER-G {a}; THAMM-P; WUENSCH-E; ERSPAMER-V
AD:  {a} MAX-PLANCK-INSTITUT FUER BIOCHEMIE, ABTEILUNG PEPTIDCHEMIE, 8033 MARTINSRIED, FRG
BK:  VOELTER, W., ET AL. (ED.). CHEMISTRY OF PEPTIDES AND PROTEINS, VOL. 3; FIFTH USSR-FRG SYMPOSIUM, ODESSA, USSR, MAY 16-20, 1985. X+414P. WALTER DE GRUYTER AND CO.: BERLIN, WEST GERMANY; WALTER DE GRUYTER, INC.: HAWTHORNE, N.Y., USA. ILLUS. 1986; : 43-48.
PY:  1986
DT:  Meeting-; Book-
IB:  3110106132; 0899251935
LA:  ENGLISH
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  FROG TOAD MAMMAL SKIN-GUT-BRAIN PEPTIDE TRIANGLE
MJCC:  CC17002 (Endocrine-System-General)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10504 (Biophysics-General-Biophysical-Techniques)
BC:  BC85306 Salientia
AN:  198732027059
UD:  1987

Record 16 of 37 in BIOSIS Previews 1987 Part 1

TI:  INDOLE IMIDAZOLE AND PHENYL-ALKYLAMINES IN THE SKIN OF ONE HUNDRED AND FORTY AMERICAN AMPHIBIAN SPECIES OTHER THAN BUFONIDS.
AU:  ROSEGHINI-M {a}; ERSPAMER-V; ERSPAMER-G-F; CEI-J-M
AD:  {a} INST MED PHARMACOL, 1ST UNIV ROME, I-00100 ROME, ITALY
SO:  Comparative-Biochemistry-and-Physiology-C-Pharmacology-Toxicology-and-Endocrinology. 1986; 85 (1): 139-148.
PY:  1986
DT:  Article-
IS:  0742-8413
LA:  ENGLISH
AB:  1. Extracts prepared from dried or fresh skins of 140 American amphibian species, other than bufonids, were subjected to chemical and biological screening in order to determine the presence and concentrations of aromatic biogenic amines. 2. The most frequent and abundantly occurring amine category was that of indolealkylamines, represented by their prototype 5-hydroxytryptamine and its N-methylated derivatives. Conjugated and cyclized indolealkylamines, typical for the toad skin, were  apparently lacking. 3. Phenylalkylamines were represented by two quaternary ammonium bases: leptodactyline and, very rarely, candicine. Leptodactyline was particularly abundant in leptodactylid frogs of the genus Leptodactylus. 4. Histamine occurred in trace amounts in different species, in large amounts only in some Leptodactylus species of the "pachypus" section. On the other hand, N-methylated histamines and cyclized histamines (spinaceamines) were confined to the skin of Leptodactylus p!
entadactylus labyrinthicus 5. The possible taxonomical and evolutionary significance of amphibian skin amines is pointed out.
AI:  Y
ST:  Amphibia-: Vertebrata-, Chordata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  LEPTODACTYLUS-PENTADACTYLUS-LABYRINTHICUS 5 HYDROXYTRYPTAMINE LEPTODACTYLINE CANDICINE HISTAMINE AROMATIC BIOGENIC AMINES TAXONOMY EVOLUTION
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10010 (Comparative-Biochemistry-General)
CC13002 (Metabolism-General-Metabolism-Metabolic-Pathways)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC62514 (Chordata-General-and-Systematic-Zoology-Amphibia)
MNCC:  CC00504 (General-Biology-Taxonomy-Nomenclature-and-Terminology)
CC01500 (Evolution)
CC10060 (Biochemical-Studies-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
BC:  BC85300 Amphibia-Unspecified; BC85306 Salientia
AN:  198783034349
UD:  1987

Record 17 of 37 in BIOSIS Previews 1987 Part 1

TI:  ACTIVE PEPTIDES IN THE SKINS OF TWO HUNDRED AND THIRTY AMERICAN AMPHIBIAN SPECIES.
AU:  ERSPAMER-V {a}; ERSPAMER-G-F; CEI-J-M
AD:  {a} INST MED PHARMACOL, 1ST UNIV ROME, I 00100 ROME, ITALY
SO:  Comparative-Biochemistry-and-Physiology-C-Pharmacology-Toxicology-and-Endocrinology. 1986; 85 (1): 125-138.
PY:  1986
DT:  Article-
IS:  0742-8413
LA:  ENGLISH
AB:  1. Extracts prepared from dried or fresh skins of more than 200 American amphibian species were subjected to biological screening in order to determine occurrence and contents of peptides active on smooth muscle preparations, systemic blood pressure and, subordinately, external secretions, anterior pituitary and the central nervous system. 2. The peptide families identified in skin extracts were as follows: caruleins (caerulein, phyllocaerulein), tachykinins (physalaemin, phyllomedusin), bombesins (phyllolitorin, [Leu8]phyllolitorin, rohdeilitorin), bradykinins (phyllokinin and others), sauvagine, dermorphins (dermorphin, [Hyp6]dermorphin), tryptophyllins (numerous peptides) and, finally, miscellaneous peptides. None of the above peptide families showed a widespread distribution, but all were restricted to particular amphibian genera or stocks. 3. The hylid frogs of the Phyllomedusinae family occupy a unique position, as their skin displayed the greatest variety and  abun!
dance of active peptides ever found in any amphibian stock in the world. 4. The array of peptide molecules occurring in the skin of the American amphibians is destined to increase because numerous other peptide molecules await isolation, elucidation of structure and definition of possible biological activities.
AI:  Y
ST:  Amphibia-: Vertebrata-, Chordata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  HYLID FROG CAERULEIN PHYLLOCAERULEIN PHYSALAEMIN PHYLLOMEDUSIN TACHYKININS PHYLLOLITORIN ROHDEILITORIN BOMBESINS PHYLLOKININ BRADYKININ SAUVAGINE DERMORPHINS TRYPTOPHYLLINS SMOOTH MUSCLE SYSTEMIC BLOOD PRESSURE EXTERNAL SECRETIONS ANTERIOR PITUITARY GLAND CENTRAL NERVOUS SYSTEM KIDNEY
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10010 (Comparative-Biochemistry-General)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC17002 (Endocrine-System-General)
CC62514 (Chordata-General-and-Systematic-Zoology-Amphibia)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC15001 (Blood-Blood-Forming-Organs-and-Body-Fluids-General-Methods)
CC15501 (Urinary-System-and-External-Secretions-General-Methods)
CC17014 (Endocrine-System-Pituitary)
CC17501 (Muscle-General-Methods)
CC20501 (Nervous-System-General-Methods)
CC32600 (In-Vitro-Studies-Cellular-and-Subcellular)
BC:  BC85300 Amphibia-Unspecified; BC85306 Salientia
AN:  198783034348
UD:  1987

Record 18 of 37 in BIOSIS Previews 1987 Part 2

TI:  RECEPTORS AND STRUCTURE-RELATED ACTIVITY OF BOMBESIN FAMILY PEPTIDES ON ISOLATED GASTRIC SMOOTH CELLS.
AU:  SEVERI-C {a}; DELLE-FAVE-G; ERSPAMER-G-F; ERSPAMER-V; TORSOLI-A
AD:  {a} GASTROENTEROL UNIT, UNIV LA SAPIENZA, ROME, ITALY
SO:  Gastroenterology-. 1987; 92 (5 PART 2): 1634.
PY:  1987
DT:  Meeting-
IS:  0016-5085
MT:  88TH ANNUAL MEETING OF THE AMERICAN GASTROENTEROLOGICAL ASSOCIATION AND DIGESTIVE DISEASE WEEK, CHICAGO, ILLINOIS, USA, MAY 9-15, 1987. GASTROENTEROLOGY
LA:  ENGLISH
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  ABSTRACT GUINEA-PIG
MJCC:  CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC17504 (Muscle-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
BC:  BC86300 Caviidae
AN:  198733064798
UD:  1987

Record 19 of 37 in BIOSIS Previews 1988 Part 1

TI:  DISCOVERY ISOLATION AND CHARACTERIZATION OF BOMBESIN-LIKE PEPTIDES.
AU:  ERSPAMER-V {a}
AD:  {a} IST FARMACOL MED, UNIV "LA SAPIENZA" ROMA, ROME, ITALY
SO:  Regulatory-Peptides. 1987; 19 (1-2): 107.
PY:  1987
DT:  Meeting-
IS:  0167-0115
MT:  INTERNATIONAL SYMPOSIUM ON BOMBESIN-LIKE PEPTIDES IN HEALTH AND DISEASE, ROME, ITALY, OCTOBER 13-16, 1987. REGUL PEPT
LA:  ENGLISH
ST:  Amphibia-: Vertebrata-, Chordata-, Animalia-; Aves-: Vertebrata-, Chordata-, Animalia-; Mammalia-: Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Birds-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Vertebrates-
MI:  ABSTRACT AMPHIBIAN BIRD MAMMAL GALL BLADDER SMOOTH MUSCLE GASTRIC ACID
MJCC:  CC17002 (Endocrine-System-General)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17020 (Endocrine-System-Neuroendocrinology)
CC17504 (Muscle-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
BC:  BC85300 Amphibia-Unspecified; BC85500 Aves-Unspecified; BC85700 Mammalia-Unspecified
AN:  198834062760
UD:  1988

Record 20 of 37 in BIOSIS Previews 1988 Part 2

TI:  A SYNTHETIC BOMBESIN-ANALOGUE ACTING AS SPECIFIC BOMBESIN RECEPTOR ANTAGONIST.
AU:  SEVERI-C {a}; ANANIA-C; COY-D-H; JENSEN-R-T; BOSCHERO-L; ERSPAMER-V; DELLE-FAVE-G
AD:  {a} GASTROENTEROL UNIT, UNIV "LA SAPIENZA", ROME, ITALY
SO:  Pharmacological-Research-Communications. 1988; 20 (SUPPL. 2): 357.
PY:  1988
DT:  Meeting-
IS:  0031-6989
MT:  XXIVTH NATIONAL CONGRESS OF THE ITALIAN SOCIETY OF FARMACOLOGY, BRESCIA, ITALY, SEPTEMBER 25-28, 1988. PHARMACOL RES COMMUN
LA:  ENGLISH
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  ABSTRACT AUTONOMIC-DRUG GUINEA-PIG MUSCLE CELL SUBSTANCE P PHARMACODYNAMICS
MJCC:  CC22024 (Pharmacology-Neuropharmacology)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC11108 (Anatomy-and-Histology-General-and-Comparative-Microscopic-and-Ultramicroscopic-Anatomy)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17504 (Muscle-Physiology-and-Biochemistry)
CC22003 (Pharmacology-Drug-Metabolism-Metabolic-Stimulators)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10060 (Biochemical-Studies-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC32500 (Tissue-Culture-Apparatus-Methods-and-Media)
BC:  BC86300 Caviidae
AN:  198835116509
UD:  1988

Record 21 of 37 in BIOSIS Previews 1988 Part 2

TI:  PARALLEL BIOASSAY OF 27 BOMBESIN-LIKE PEPTIDES ON 9 SMOOTH MUSCLE PREPARATIONS STRUCTURE-ACTIVITY RELATIONSHIPS AND BOMBESIN RECEPTOR SUBTYPES.
AU:  FALCONIERI-ERSPAMER-G {a}; SEVERINI-C; ERSPAMER-V; MELCHIORRI-P; DELLE-FAVE-G; NAKAJIMA-T
AD:  {a} INST PHARMACOL PHARMACOGNOSY, UNIV ROME 'LA SAPIENZA', CITTA UNIVERSITARIA, 00100 ROME, ITALY
SO:  Regulatory-Peptides. 1988; 21 (1-2): 1-12.
PY:  1988
DT:  Article-
IS:  0167-0115
LA:  ENGLISH
AB:  Thirteen natural bombesin-like peptides and 14 synthetic analogues were submitted to parallel bioassay on 9 smooth muscle preparations in order to determine their relative potency, in comparison to bombesin and litorin. The natural peptides of the bombesin subfamily showed a uniformly high or moderate potency on all preparations. However, synthetic bombesins of shorter chain length (hepta- and octapeptides) manifested a good potency only on the rat uterus preparation. Among the peptides of the  litorin and phyllolitorin subfamilies, only litorin and ranatensin presented a full spectrum of potency, equalling or even surpassing that of bombesin. All other natural and synthetic members of the two subfamilies showed a sharply dissociated spectrum of potency on the different smooth muscle preparations. The only exception was the rat urinary bladder and, in part, the chicken intestine, on which the peptides displayed a uniformly high potency, comparable to, or even greater than!
 that of  bombesin. The present results help to explain structure/activity relationships and suggest the probable existence, in the periphery, of multiple bombesin receptor subtypes.
AI:  Y
ST:  Galliformes-: Aves-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Birds-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  RAT CHICKEN SMOOTH MUSCLE CONTRACTION UTERINE BLADDER INTESTINE
MJCC:  CC22022 (Pharmacology-Muscle-System)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC12003 (Physiology-General-and-Miscellaneous-Comparative)
CC13012 (Metabolism-Proteins-Peptides-and-Amino-Acids)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC15504 (Urinary-System-and-External-Secretions-Physiology-and-Biochemistry)
CC16504 (Reproductive-System-Physiology-and-Biochemistry)
CC17504 (Muscle-Physiology-and-Biochemistry)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC11104 (Anatomy-and-Histology-General-and-Comparative-Experimental-Anatomy)
BC:  BC85536 Galliformes; BC86375 Muridae
AN:  198886041280
UD:  1988

Record 22 of 37 in BIOSIS Previews 1988 Part 2

TI:  PSEUDOPHRYNAMINE A AN UNUSUAL PRENYL PYRROLO-2 3-B-INDOLE ESTER FROM AN AUSTRALIAN FROG PSEUDOPHRYNE-CORIACEA MYOBATRACHIDAE.
AU:  SPANDE-T-F {a}; EDWARDS-M-W; PANNELL-L-K; DALY-J-W; ERSPAMER-V; MELCHIORRI-P
AD:  {a} LAB BIOORGANIC CHEM, NATL INST DIABETES AND DIGESTIVE AND KIDNEY DISEASES, NATL INST HEALTH, BETHESDA, MARYLAND 20892
SO:  Journal-of-Organic-Chemistry. 1988; 53 (6): 1222-1226.
PY:  1988
DT:  Article-
IS:  0022-3263
LA:  ENGLISH
AB:  Three new alkaloids having a tricyclic N-methyltryptamine of the physostigmine type and an isoprene-derived side chain have been isolated from the skins of the Australian frog Pseudophryne coriacea and their structures determined by mass spectrometry, NMR spectroscopy, and chemical interconversions.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MJCC:  CC54000 (Pharmacognosy-and-Pharmaceutical-Botany)
CC10060 (Biochemical-Studies-General)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC10050 (Biochemical-Methods-General)
BC:  BC85306 Salientia
AN:  198886007367
UD:  1988

Record 23 of 37 in BIOSIS Previews 1989 Part 1

TI:  DELTORPHIN A NOVEL AMPHIBIAN SKIN PEPTIDE WITH HIGH SELECTIVITY AND AFFINITY FOR DELTA OPIOID RECEPTORS.
AU:  KREIL-G {a}; BARRA-D; SIMMACO-M; ERSPAMER-V; ERSPAMER-G-F; NEGRI-L; SEVERINI-C; CORSI-R; MELCHIORRI-P
AD:  {a} INST MED PHARMACOL III, UNIV ROME 'LA SAPIENZA', PZA A MORO 5, 00185 ROME, ITALY
SO:  European-Journal-of-Pharmacology. 1989; 162 (1): 123-128.
PY:  1989
DT:  Article-
IS:  0014-2999
LA:  ENGLISH
AB:  With a cDNA library prepared from skin of Phyllomedusa sauvagei, the sequence of the precursor of dermorphin was elucidated recently. The sequence suggested the existence of another peptide, distantly related to dermorphin. Two variants of this peptide have now been synthesized containing either L- or D-methionine as the second amino acid. The peptide containing the D-methionine exhibited high-affinity and selectivity for .delta. opioid receptors in the mouse vas deferens and in rat brain  homogenates. Moreover, using the synthetic peptide as marker, we could isolate small quantities of the corresponding natural peptide containing D-methionine as the second amino acid from skin extracts of P. sauvagei. The name deltorphin is proposed for this new peptide and its sequence is Tyr-D-Met-Phe-His-Leu-Met-Asp-NH2.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PHYLLOMEDUSA-SAUVAGEI MOUSE RAT BRAIN VAS DEFERENS AMINO ACID SEQUENCE MOLECULAR SEQUENCE DATA
MJCC:  CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10508 (Biophysics-Membrane-Phenomena)
CC16504 (Reproductive-System-Physiology-and-Biochemistry)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
BC:  BC85306 Salientia
AN:  198987129007
UD:  1989

Record 24 of 37 in BIOSIS Previews 1989 Part 1

TI:  A NATURALLY OCCURRING ERYTHRO DIASTEREOMER OF PUMILIOTOXIN B.
AU:  GARRAFFO-H-M {a}; EDWARDS-M-W; SPANDE-T-F; DALY-J-W; OVERMAN-L-E; SEVERINI-C; ERSPAMER-V
AD:  {a} LAB BIOORGANIC CHEM, BUILD 8, ROOM 1A17, NATL INST ARTHRITIS DIABETES DIG KIDNEY DIS, NATL INST HEALTH, BETHESDA, MD 20892
SO:  Tetrahedron-. 1988; 44 (22): 6795-6800.
PY:  1988
DT:  Article-
IS:  0040-4020
LA:  ENGLISH
AB:  The 15R, 16S erythro diastereomer of pumiliotoxin B has been discovered in the skin of an Austrialian myobatrachid frog, Pseudophryne coriacea.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  DENDROBATES-PUMILIO PSEUDOPHRYNE-CORIACEA NMR ANALYSIS
MJCC:  CC22501 (Toxicology-General-Methods-and-Experimental)
CC10060 (Biochemical-Studies-General)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC10504 (Biophysics-General-Biophysical-Techniques)
CC18501 (Integumentary-System-General-Methods)
BC:  BC85306 Salientia
AN:  198987065867
UD:  1989

Record 25 of 37 in BIOSIS Previews 1989 Part 2

TI:  DELTORPHINS A FAMILY OF NATURALLY OCCURRING PEPTIDES WITH HIGH AFFINITY AND SELECTIVITY FOR DELTA OPIOID BINDING SITES.
AU:  ERSPAMER-V {a}; MELCHIORRI-P; FALCONIERI-ERSPAMER-G; NEGRI-L; CORSI-R; SEVERINI-C; BARRA-D; SIMMACO-M; KREIL-G
AD:  {a} INST PHARMACOL, UNIV LA SAPIENZA, 00185 ROME, ITALY
SO:  Proceedings-of-the-National-Academy-of-Sciences-of-the-United-States-of-America. 1989; 86 (13): 5188-5192.
PY:  1989
DT:  Article-
IS:  0027-8424
LA:  ENGLISH
AB:  Deltorphins are endogenous linear heptapeptides, isolated from skin extracts of frogs belonging to the genus Phyllomedusa, that have a higher affinity and selectively for .delta. opioid binding sites than any other natural compound known. Two deltorphins with the sequence Tyr-Ala-Phe-Asp(or Glu)-Val-Val-Gly-NH2 have been isolated from skin extracts of Phyllomedusa bicolor. The alanine in position 2 is in the D configuration. These peptides, [D-Ala2]deltorphins I and II, show an even higher affinity for .delta. receptors than the previously characterized deltorphin, which contains D-methionine as the second amino acid. These peptides show some similarity to another constituent of Phyllomedusa skin, dermorphin, which is highly selective for .mu.-opioid receptors. These peptides all have the N-terminal sequence Tyr-D-Xaa-Phe, where D-Xaa is either D-alanine or D-methionine. While this structure seems to be capable of activating both .mu. and .delta. opioid receptors, differe!
nces in the  C-terminal regions of these peptides are probably responsible for the observed high receptor selectivity of dermorphin and deltorphin.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PHYLLOMEDUSA-BICOLOR SKIN MU RECEPTOR SELECTIVE DERMORPHIN MOLECULAR SEQUENCE DATA AMINO ACID SEQUENCE
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC10508 (Biophysics-Membrane-Phenomena)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC10010 (Comparative-Biochemistry-General)
BC:  BC85306 Salientia
AN:  198988062679
UD:  1989

Record 26 of 37 in BIOSIS Previews 1989 Part 2

TI:  ISOLATION STRUCTURE DETERMINATION AND SYNTHESIS OF A NOVEL TRYPTOPHAN-CONTAINING HEPTAPEPTIDE BASIC TRYPTOPHYLLIN FROM THE SKIN OF PHYLLOMEDUSA-ROHDEI.
AU:  MONTECUCCHI-P-C {a}; VINCENTI-M; LAZZARINI-A-M; ROSCONI-L; ERSPAMER-V
AD:  {a} FARMITALIA CARLO ERVA, VIA DEI GRACCHI 35, 20146 MILAN, ITALY
SO:  International-Journal-of-Peptide-and-Protein-Research. 1989; 33 (5): 391-395.
PY:  1989
DT:  Article-
IS:  0367-8377
LA:  ENGLISH
AB:  A novel tryptophyllin has been isolated from the methanol extracts of the skin of the South American frog Phyllomedusa rohdei. Its primary structure, as determined by chemical/enzymatic methods and by FAB mass spectrometry, is the following: Lys-Pro-Hyp-Ser-Trp-Ile-Pro-NH2. This basic tryptophyllin has been synthesized in solid phase, in order to allow its complete pharmacological characterization.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  SOLID PHASE PEPTIDE SYNTHESIS
MJCC:  CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
BC:  BC85306 Salientia
AN:  198988022227
UD:  1989

Record 27 of 37 in BIOSIS Previews 1989 Part 2

TI:  PUMILIOTOXIN B-LIKE ALKALOID IN EXTRACTS OF THE SKIN OF THE AUSTRALIAN MYOBATRACHID FROG PSEUDOPHRYNE-CORIACEA EFFECTS ON THE SYSTEMIC BLOOD PRESSURE OF EXPERIMENTAL ANIMALS AND THE RAT HEART.
AU:  ERSPAMER-G-F {a}; SEVERINI-C; ERSPAMER-V; MELCHIORRI-P
AD:  {a} INST MEDICAL PHARMACOLOGY III, UNIV ROME "LA SAPIENZA", CITTA UNIVERSITARIA, 00100 ROME, ITALY
SO:  Neuropharmacology-. 1989; 28 (4): 319-328.
PY:  1989
DT:  Article-
IS:  0028-3908
LA:  ENGLISH
AB:  Semi-purified extracts of the skin of the Australian myobatrachid frog Pseudophyryne coriacea (PS) displayed striking, reversible and, in part, dose-dependent effects on the systemic blood pressure of the rat and other experimental animals, as well as on the rat heart. The blood pressure response in the rat consisted typically in an abrupt, short-lasting fall, followed by a conspicuous, more persistent rise. The fall in pressure was abolished by atropine and potentiated by physostigmine,  indicating a cholinergic mechanisms; rise was abolished by prazosin and guanethidine, suggesting a release of catecholamines from adrenergic nerve terminals in the vasculature. Tachyphylaxis was the obvious result of exhaustion of stores of catecholamines. On the heart, as shown in the electrocardiographic tracings, PS produced a variety of rhythm disorders, attributable both to the release of aminergic transmitters and to a direct effect on the myocardium. Whereas the pressure and  elec!
trocardiographic responses were virtually unaffected by calcium channel antagonists and agonists, all the effects of PS were sharply reduced or completely abolished by the sodium channel blocker tetrodotoxin. This suggests that activation of sodium channels may play a role in the mechanism of action of PS. However, the exact nature of ionic flux(es) influenced by the extract of frog skin remains to be established.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  AUTONOMIC-DRUG PHARMACODYNAMICS BLOOD PRESSURE RHYTHM DISORDER AMINERGIC TRANSMITTER ELECTROCARDIOGRAM
MJCC:  CC22024 (Pharmacology-Neuropharmacology)
CC12004 (Physiology-General-and-Miscellaneous-Instrumentation)
CC14504 (Cardiovascular-System-Physiology-and-Biochemistry)
CC14506 (Cardiovascular-System-Heart-Pathology)
CC15002 (Blood-Blood-Forming-Organs-and-Body-Fluids-Blood-and-Lymph-Studies)
CC17504 (Muscle-Physiology-and-Biochemistry)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC22003 (Pharmacology-Drug-Metabolism-Metabolic-Stimulators)
CC22010 (Pharmacology-Cardiovascular-System)
MNCC:  CC10504 (Biophysics-General-Biophysical-Techniques)
CC14501 (Cardiovascular-System-General-Methods)
CC22005 (Pharmacology-Clinical-Pharmacology)
CC28002 (Laboratory-Animals-General)
BC:  BC85306 Salientia; BC86375 Muridae
AN:  198988019019
UD:  1989

Record 28 of 37 in BIOSIS Previews 1990 Part 1

TI:  BRAIN-GUT-SKIN PEPTIDES AN UPDATE OVERVIEW.
AU:  RENDA-T {a}; D'-ESTE-L; FASOLO-A; LAZARUS-L-H; MINNITI-F; ERSPAMER-V
AD:  {a} IST DI ANATOMIA UMANA NORMALE, UNIV "LA SAPIENZA", VIALE REGINA ELENA 289, 00161 ROMA, ITALY
SO:  Archives-of-Histology-and-Cytology. 1989; 52 (SUPPL): 317-324.
PY:  1989
DT:  Meeting-
IS:  0914-9465
MT:  INTERNATIONAL SYMPOSIUM ON NEURONS AND PARANEURONS, NIIGATA, JAPAN, OCTOBER 26-29, 1988. ARCH HISTOL CYTOL
LA:  ENGLISH
ST:  Pisces-: Vertebrata-, Chordata-, Animalia-; Amphibia-: Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Fish-; Nonhuman-Vertebrates; Vertebrates-
MI:  REVIEW FISH AMPHIBIAN MAMMAL SAUVAGINE TACHYKININ BOMBESIN DERMORPHIN
MJCC:  CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC17002 (Endocrine-System-General)
CC17020 (Endocrine-System-Neuroendocrinology)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10010 (Comparative-Biochemistry-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC12003 (Physiology-General-and-Miscellaneous-Comparative)
BC:  BC85200 Pisces-Unspecified; BC85300 Amphibia-Unspecified
AN:  199038058516
UD:  1990

Record 29 of 37 in BIOSIS Previews 1990 Part 1

TI:  SIX NOVEL TACHYKININ-RELATED AND BOMBESIN-RELATED PEPTIDES FROM THE SKIN OF THE AUSTRALIAN FROG PSEUDOPHRYNE-GUNTHERI.
AU:  SIMMACO-M {a}; SEVERINI-C; DE-BIASE-D; BARRA-D; BOSSA-F; ROBERTS-J-D; MELCHIORRI-P; ERSPAMER-V
AD:  {a} DIPARTIMENTO DI SCI BIOCHIMICHE, UNIV LA SAPIENZA, PIAZZALE ALDO MORO 5, 00185 ROMA, ITALIA
SO:  Peptides-New-York. 1990; 11 (2): 299-304.
PY:  1990
DT:  Article-
IS:  0196-9781
LA:  ENGLISH
AB:  Six novel peptides belonging to the tachykinin and bombesin families were isolated and sequenced from extracts of the skin of the Australian myobatrachid frog Pseudophryne guntheri. One of these peptides (PG-L) was of the bombesin family and may be considered an N-elongation of the litorin/ranatensin molecule, with which it shares an identical spectrum of activity on isolated smooth muscle preparations. The other five peptides were of the tachykinin family with two of these peptides (PG-SPI  and PG-SPII) related to substance P and three (PG-KI, PG-KII and PG-KIII) to kassinin. In contrast to the basic nature of substance P, the PG-SP peptides showed a clear acidic character and displayed a more potent and sustained action on isolated smooth muscle preparations and rat blood pressure than did substance P. Two of the three PG-K peptides were more potent than kassinin; PG-KIII was considerably less potent. PG-KI and PG-KII were also present in a deamidated, poorly active, fo!
rm.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  LITORIN-LIKE PEPTIDES SUBSTANCE P-LIKE PEPTIDES KASSININ-LIKE PEPTIDES PRIMARY STRUCTURE BIOACTIVITY
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC10054 (Biochemical-Methods-Proteins-Peptides-and-Amino-Acids)
CC18501 (Integumentary-System-General-Methods)
BC:  BC85306 Salientia
AN:  199089127144
UD:  1990

Record 30 of 37 in BIOSIS Previews 1990 Part 2

TI:  ALKALOIDS FROM BUFONID TOADS OF THE GENUS MELANOPHRYNISCUS.
AU:  GARRAFFO-H-M {a}; SPANDE-T-F; DALY-J-W; SEVERINI-C; ERSPAMER-V; BALDESSARI-A; GROS-E-G
AD:  {a} NIH, BETHESDA, MD 20892
SO:  Abstracts-of-Papers-American-Chemical-Society. 1990; 200 (1-2): AGFD 41.
PY:  1990
DT:  Meeting-
IS:  0065-7727
MT:  200TH AMERICAN CHEMICAL SOCIETY NATIONAL MEETING, WASHINGTON, D.C., USA, AUGUST 26-31, 1990. ABSTR PAP AM CHEM SOC
LA:  ENGLISH
ST:  Insecta-: Arthropoda-, Invertebrata-, Animalia-; Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Arthropods-; Chordates-; Insects-; Invertebrates-; Nonhuman-Vertebrates; Vertebrates-
MI:  ABSTRACT MELANOPHRYNISCUS-STELZNERI MELANOPHRYNISCUS-STELZNERI-MONTEVIDENSIS INSECT DENDROBATID PUMILIOTOXIN 251D HOMOPUMILOTOXIN INDOLIZIDINE PYRROLIZIDINE QUINOLIZIDINE DECAHYDROQUINOLINE URUGUAY ARGENTINA
MJCC:  CC22501 (Toxicology-General-Methods-and-Experimental)
CC10010 (Comparative-Biochemistry-General)
CC10060 (Biochemical-Studies-General)
CC62514 (Chordata-General-and-Systematic-Zoology-Amphibia)
MNCC:  CC00520 (General-Biology-Symposia-Transactions-and-Proceedings-of-Conferences-Congresses-Review-Annuals)
CC10504 (Biophysics-General-Biophysical-Techniques)
CC62800 (Animal-Distribution)
BC:  BC75300 Insecta-Unspecified; BC85306 Salientia
AN:  199039101593
UD:  1990

Record 31 of 37 in BIOSIS Previews 1990 Part 2

TI:  ALKALOIDS FROM AUSTRALIAN FROGS MYOBATRACHIDAE PSEUDOPHRYNAMINES AND PUMILIOTOXINS.
AU:  DALY-J-W {a}; GARRAFFO-H-M; PANNELL-L-K; SPANDE-T-F; SEVERINI-C; ERSPAMER-V
AD:  {a} LAB BIOORG CHEM, BUILD 8, ROOM 1A17, NATL INST DIABETES DIG KIDNEY DIS, NATL INST HEALTH, BETHESDA, MD 20892, USA
SO:  Journal-of-Natural-Products-Lloydia. 1990; 53 (2): 407-421.
PY:  1990
DT:  Article-
IS:  0163-3864
LA:  ENGLISH
AB:  Australian frogs of the genus Pseudophryne contain two distinct classes of alkaloids. The pseudophrynamine class (3a-prenyl pyrrolo[2,3-b]indoles) are unique to this genus of frogs of the family Myobatrachidae, while the pumiliotoxin-A class (8-hydroxy-8-methyl-6-alkylidene-1-azabicyclo[4.3.0]nonanes) also occur in dendrobatid frogs of the genera Dendrobates, Epipedobates, and Minyobates, in ranid frogs of the genus Mantella, and in bufonid toads of the genus Melanophryniscus. All seven species of Pseudophryne examined contain both classes of alkaloids. The pseudophrynamines were the predominant class in both species (Pseudophyrine guentheri and Pseudophryne occidentalis) from Western Australia, while all of the eastern species (Pseudophryne australis, Pseudophryne bibronii, Pseudophryne coriacea, Pseudophryne corroboree, and Pseudophryne semiarmorata) contained significant amounts of both pseudophrynamines and pumiliotoxins. Pumiliotoxins, in particular pumiliotoxin B, w!
ere predominant in two eastern species (P. australis and a southern population of P. corroboree), while pseudophyrnamines were dominant in P. bibronii, four of six populations of P. coriacea, one population of P. semimarmorata, and a northern population of P. corroboree. Structures are proposed for several new alkaloids of the pseudophrynamine class.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  PSEUDOPHRYNE-GUENTHERI PSEUDOPHRYNE-OCCIDENTALIS PSEUDOPHRYNE-AUSTRALIS PSEUDOPHRYNE-BIBRONII PSEUDOPHRYNE-CORIACEA PSEUDOPHRYNE-CORROBOREE PSEUDOPHRYNE-SEMIMARMORATA DENDROBATES EPIPEDOBATES MINYOBATES MANTELLA MELANOPHRYNISCUS SKIN SECRETIONS
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10060 (Biochemical-Studies-General)
CC22501 (Toxicology-General-Methods-and-Experimental)
BC:  BC85306 Salientia
AN:  199090051589
UD:  1990

Record 32 of 37 in BIOSIS Previews 1991 Part 1

TI:  FACILITATION OF TRITIATED ACETYLCHOLINE AND TRITIATED 5 HYDROXYTRYPTAMINE RELEASE FROM RAT CEREBRAL CORTEX SYNAPTOSOMES BY A FACTOR EXTRACTED FROM THE SKIN OF THE AUSTRALIAN FROG PSEUDOPHRYNE-CORIACEA.
AU:  RAITERI-M {a}; MARCHI-M; BONANNO-G; MELCHIORRI-P; ERSPAMER-V
AD:  {a} IST FARMACOL E FARMACOGNOSIA, VIALE CEMBRANO 4, 16148 GENOVA, ITALY
SO:  European-Journal-of-Pharmacology-Molecular-Pharmacology-Section. 1989; 1 (3): 223-230.
PY:  1989
DT:  Article-
IS:  0922-4106
LA:  ENGLISH
AB:  A partly purified extract of the skin of the Australian frog Pseudophryne coriacea (PsC) evoked the release of [3H]acetylcholine ([3H]ACh) and of [3H]5-hydroxytryptamine ([3H]5-HT) from superfused rat cerebral cortex synaptosomes prelabeled with [3H]choline or [3H]5-HT, respectively. The PsC-evoked release of both transmitters was sensitive to tetrodotoxin and was strictly Ca2+-dependent. The release of [3H]5-HT caused by PsC was unaffected by the 5-HT uptake inhibitor citalopram. Activation of muscarinic autoreceptors by ACh or of serotonin autoreceptors by 5-HT depressed the PsC-evoked release of [3H]ACh or of [3H]5-HT, respectively. It is concluded that PsC elicits a Ca2+-dependent exocytotic-like transmitter release, possibly by opening Na+ channels in the presynaptic membrane.
AI:  Y
ST:  Salientia-: Amphibia-, Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Nonhuman-Vertebrates; Vertebrates-
MI:  SODIUM CHANNEL
MJCC:  CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10508 (Biophysics-Membrane-Phenomena)
CC13010 (Metabolism-Minerals)
CC17020 (Endocrine-System-Neuroendocrinology)
MNCC:  CC10069 (Biochemical-Studies-Minerals)
BC:  BC85306 Salientia
AN:  199191041942
UD:  1991

Record 33 of 37 in BIOSIS Previews 1991 Part 2

TI:  DIFFERENT RECEPTORS MEDIATE THE ACTION OF BOMBESIN-RELATED PEPTIDES ON GASTRIC SMOOTH MUSCLE CELLS.
AU:  SEVERI-C {a}; JENSEN-R-T; ERSPAMER-V; D'-ARPINO-L; COY-D-H; TORSOLI-A; DELLE-FAVE-G
AD:  {a} CATTEDRA GASTROENTEROLOGIA 1, CLINICA MEDICA 2, POLICLINICO UMBERTO I, VIALE DE POLICLINICO, 00161 ROME, ITALY
SO:  American-Journal-of-Physiology. 1991; 260 (5 PART 1): G683-G690.
PY:  1991
DT:  Article-
IS:  0002-9513
LA:  ENGLISH
AB:  Recent studies suggest that different subtypes of receptors may mediate the action of various bombesin-related peptides in different tissues. In the present study the ability of bombesin and its structurally related peptides {litorin, gastrin-releasing peptide (GRP), GRP18-27, neuromedin B, [Leu8]litorin, and bombesin nonapeptide BN(6-14)} to interact with smooth muscle cells isolated from guinea pig stomach was investigated. Each peptide induced a specific contractile response with potencies (D50 in pM) of [Leu8]litorin (0.7) > bombesin (1.2) > litorin (3) > neuromedin B (3.5) = GRP (3.8) = GRP18-27 (3.9) > BN(6-14) (70.9). The specific bombesin receptor antagonist .phi.13,14-bomesin differed in its potency for inhibiting equipotent concentrations of bombesin, GRP, or neuromedin B, was equipotent for bombesin or GRP (IC50 12.7 and 22.1 nM), and was 11 times less potent for neuromedian B (IC50 234.5 nM), suggesting the presence of subtypes of  receptors mediating the acti!
on of bombesin-related peptides. To further investigate this possibility, a technique of receptor protection that enables selective preservation of one receptor type was used. GRP or bombesin protected completely the response to GRP or bombesin but abolished the subsequent contractile response to neuromedin B. Neuromedin B, instead, protected only the response to neuromedin B. These results demonstrate that gastric smooth muscle cells possess specific receptors that  interact with bombesin-related peptides and that two receptor subtypes mediate the contractile response to these peptides: one subtype is selective for bombesin or GRP, the other for neuromedin B.
AI:  Y
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  GUINEA-PIG GASTRIN-RELEASING PEPTIDE NEUROMEDIN B BOMBESIN RECEPTOR SUBTYPES CONTRACTILE RESPONSE
MJCC:  CC14004 (Digestive-System-Physiology-and-Biochemistry)
CC02506 (Cytology-and-Cytochemistry-Animal)
CC10508 (Biophysics-Membrane-Phenomena)
CC14002 (Digestive-System-Anatomy)
CC17002 (Endocrine-System-General)
CC17502 (Muscle-Anatomy)
CC17504 (Muscle-Physiology-and-Biochemistry)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
BC:  BC86300 Caviidae
AN:  199192026882
UD:  1991

Record 34 of 37 in BIOSIS Previews 1991 Part 2

TI:  STRUCTURE-ACTIVITY RELATIONSHIPS OF THE DELTA OPIOID-SELECTIVE AGONISTS DELTORPHINS.
AU:  MELCHIORRI-P {a}; NEGRI-L; FALCONIERI-ERSPAMER-G; SEVERINI-C; CORSI-R; SOAJE-M; ERSPAMER-V; BARRA-D
AD:  {a} INST PHARMACOLOGY, UNIVERSITY 'LA SAPIENZA', PIAZZA A MORO 5, 00185 ROME, ITALY
SO:  European-Journal-of-Pharmacology. 1991; 195 (2): 201-208.
PY:  1991
DT:  Article-
IS:  0014-2999
LA:  ENGLISH
AB:  Deltorphins are naturally occurring peptides with high affinity and selectivity for .delta.-opioid receptors. They share with dermorphin, another .mu.-selective opioid agonist, the same N-terminal tripeptide Tyr-D-Xaa-Phe, where D-Xaa is a D-Ala or a D-Met residue. This common sequence appears to be essential for the best fitting of the peptides to both .mu.- or .delta.-opioid sites. We studied the changes in receptor affinity and selectivity and in biological potency of deltorphins due to shortening of the  sequence, C-terminal deamidation or single amino acid substitutions. The results support the view that a code addressing the molecule towards .delta.-opioid sites is expressed in the C-terminal region of these peptides. This addressing domain confers high .delta.-selectivity to the ligand in the following two ways: (i) increased affinity for .delta.-sites; (ii) decreased affinity for .mu.-sites. The sequence of the C-terminal tripeptide appears to be responsible for t!
he high .delta.-affinity of the molecules.  Negatively charged side chains inhibit .mu.-binding and enhanced .delta.-selectivity.
AI:  Y
ST:  Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  RAT CARBOXYL-TERMINAL TRIPEPTIDE PHARMACOLOGICAL TOOL
MJCC:  CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC10508 (Biophysics-Membrane-Phenomena)
CC22024 (Pharmacology-Neuropharmacology)
BC:  BC86375 Muridae
AN:  199192007716
UD:  1991

Record 35 of 37 in BIOSIS Previews 1992 Part 2

TI:  THE OPIOID PEPTIDES OF THE AMPHIBIAN SKIN.
AU:  ERSPAMER-V {a}
AD:  {a} INSTITUTE MEDICAL PHARMACOLOGY III, UNIVERSITY "LA SAPIENZA", CITTA UNIVERSITARIA, 00100 ROME, ITALY
SO:  International-Journal-of-Developmental-Neuroscience. 1992; 10 (1): 3-30.
PY:  1992
DT:  Article-
IS:  0736-5748
LA:  ENGLISH
ST:  Amphibia-: Vertebrata-, Chordata-, Animalia-; Mammalia-: Vertebrata-, Chordata-, Animalia-
TN:  Amphibians-; Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Vertebrates-
MI:  MAMMAL DERMORPHINS DELTORPHINS AMINO ACID SEQUENCE
MJCC:  CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC17020 (Endocrine-System-Neuroendocrinology)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC10010 (Comparative-Biochemistry-General)
CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
BC:  BC85300 Amphibia-Unspecified; BC85700 Mammalia-Unspecified
AN:  199243012393
UD:  1992

Record 36 of 37 in BIOSIS Previews 1992 Part 2

TI:  DERMORPHIN-RELATED PEPTIDES FROM THE SKIN OF PHYLLOMEDUSA-BICOLOR AND THEIR AMIDATED ANALOGS ACTIVATE TWO MU OPIOID RECEPTOR SUBTYPES THAT MODULATE ANTINOCICEPTION AND CATALEPSY IN THE RAT.
AU:  NEGRI-L {a}; FALCONIERI-ERSPAMER-G; SEVERINI-C; POTENZA-R-L; MELCHIORRI-P; ERSPAMER-V
AD:  {a} INST PHARMACOL, UNIV LA SAPIENZA, 00185 ROME, ITALY
SO:  Proceedings-of-the-National-Academy-of-Sciences-of-the-United-States-of-America. 1992; 89 (15): 7203-7207.
PY:  1992
DT:  Article-
IS:  0027-8424
LA:  ENGLISH
AB:  Three naturally occurring dermorphin-like peptides from the skin of the frog Phyllomedusa bicolor, the related carboxyl-terminal amides, and some substituted analogs were synthesized, their binding profiles to opioid receptors were determined, and their biological activities were studied in isolated organ preparations and intact animals. The opioid binding profile revealed a very high selectivity of these peptides for .mu. sites and suggested the existence of two receptors subtypes, of high and low affinity. The peptides tested acted as potent .mu. opioid agonists on isolated organ preparations. They were several times more active in inhibiting electrically evoked contractions in guinea pig ileum than in mouse vas deferens. When injected into the lateral brain ventricle or peritoneum of rats, the high-affinity-site-preferring ligand, [Lys7-NH2]dermorphin, behaved as a potent analgesic agent. By contrast, the low-affinity-site-preferring ligand, [Trp4,Asn7-NH2]dermorphin, !
produced a weak antinociception but an intense catalepsy.
AI:  Y
ST:  Caviidae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-; Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Mammals; Nonhuman-Vertebrates; Rodents-; Vertebrates-
MI:  GUINEA-PIG MOUSE 7 LYSINAMIDE DERMORPHIN 4 TRYPTOPHAN-7-ASPARAGINAMIDE DERMORPHIN ANALGESIC-DRUG HIGH-AFFINITY RECEPTOR LOW-AFFINITY RECEPTOR
MJCC:  CC22024 (Pharmacology-Neuropharmacology)
CC10506 (Biophysics-Molecular-Properties-and-Macromolecules)
CC10508 (Biophysics-Membrane-Phenomena)
CC17020 (Endocrine-System-Neuroendocrinology)
CC18504 (Integumentary-System-Physiology-and-Biochemistry)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
MNCC:  CC10064 (Biochemical-Studies-Proteins-Peptides-and-Amino-Acids)
CC22016 (Pharmacology-Endocrine-System)
BC:  BC86300 Caviidae; BC86375 Muridae
AN:  199294124455
UD:  1992

Record 37 of 37 in BIOSIS Previews 1992 Part 2

TI:  LONG-TERM SENSITIZATION TO THE ACTIVATION OF CEREBRAL DELTA OPIOID RECEPTORS BY THE DELTROPHIN TYR-D-ALA-PHE-GLU-VAL-VAL-GLY-AMIDE IN RATS EXPOSED TO MORPHINE.
AU:  MELCHIORRI-P {a}; MARITATI-M; NEGRI-L; ERSPAMER-V
AD:  {a} INST MED PHARMACOL, UNIV LA SAPIENZA, 00185 ROME, ITALY
SO:  Proceedings-of-the-National-Academy-of-Sciences-of-the-United-States-of-America. 1992; 89 (9): 3696-3700.
PY:  1992
DT:  Article-
IS:  0027-8424
LA:  ENGLISH
AB:  In experiments to evaluate responses to the activation of cerebral .delta.-opioid receptors, repeated daily injection of the selective .delta.-opioid agonist Tyr-D-Ala-Phe-Glu-Val-Val-Gly-NH2 ([D-Ala2]deltorphin II) into rat brain resulted in the development of tolerance, whereas repeated daily injection or continuous infusion of morphine resulted in sensitization to the behavioral activating effects of the .delta.-opioid agonist. Although the rats did not modify their spontaneous locomotor activity after  morphine withdrawal, they became markedly hyperresponsive to the locomotor and stereotypy-producing effects of a challenge dose of the .delta.-opioid agonist. Sensitization to activation of .delta.-opioid receptors persisted for at least 60 days after discontinuing morphine treatment. These results show that the development of tolerance and long-term sensitization to opioids involves .delta.-opioid as well as .mu.-opioid receptors.
AI:  Y
ST:  Muridae-: Rodentia-, Mammalia-, Vertebrata-, Chordata-, Animalia-
TN:  Animals-; Chordates-; Mammals-; Nonhuman-Vertebrates; Nonhuman-Mammals; Rodents-; Vertebrates-
MI:  CENTRAL DEPRESSANT-DRUG PHARMACODYNAMICS TOLERANCE BEHAVIORAL EFFECT
MJCC:  CC22024 (Pharmacology-Neuropharmacology)
CC07003 (Behavioral-Biology-Animal-Behavior)
CC10508 (Biophysics-Membrane-Phenomena)
CC20504 (Nervous-System-Physiology-and-Biochemistry)
CC22003 (Pharmacology-Drug-Metabolism-Metabolic-Stimulators)
MNCC:  CC10060 (Biochemical-Studies-General)
BC:  BC86375 Muridae
AN:  199294021279
UD:  1992