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Vuillaume M, Berkaloff A. 
“LSD treatment of Pieris brassicae and consequences on the progeny”. 
Nature. 1974;251:314-315.
Abstract
At 20C, under white light illumination and circadian rhythm, nymphal diapause of Pieris brassicae is dependent on a short 9-h photophasc. Some psychodysIeptics (mescaline sulphate and LSD), when injected during the photosensitive larval period, suppress diapause induction as if the larvae were subjected to a long 16-h photophase. Abnormalities in the behaviour of the progeny of treated animals were observed. We studied them for three generations after the treatment. Three couples of P. brassicae, progeny of a single original pair, were isolated. The progeny of each of these pairs were divided into two groups, one served as control (normal stock) and the other was treated with LSD. Control and treated animals of each progeny, respectively, were pooled at the end of each experiment after assessment of the fluctuation. Both stocks were subjected to a 9-h white light photophase treatment at 20 C (14,600 erg cm' s with a is only observed if both parents were treated and completely Mazda TFR/40/BBL lamp). LSD was diluted in Ringer solution to different concentrations. Animals were injected with 5 mcl of the solution 24 h after the fourth moult. As previously described, injection of 20 mcg of LSD per animal results in about 800f continuous development and 20 0iapause induction. In the parental generation the LD10, was 35 mcg per animal. Among non-diapausing animals, different crosses were performed (Table 1). The normal partners were taken from the normal stock defined earlier. The first striking observation was that all these F. larvae were highly resistant to LSD with a LD10, higher than 60 mcg per animal. Second, 1000f the diapausing progeny of cross B, whether or not injected with 20 fig of LSD, died either at the end of the chrysalid stage or as malformed imagos Among diapausing animals, new crosses were performed (Table 1). Normal animals were taken from the progeny of the normal stock defined earlier. The sensitivity of the F. larvae to LSD in the three types of crosses seemed very high: the LSD fluctuated between 5 and 10 mcg per animal. (TEXT EDITED HERE...) ...We must then conclude that the resistance mechanism observed in the F1 is very efficient when transmitted by males and less efficient when transmitted by females. The LSD triggered enhancement of the resistance to the drug seems to be transmitted only by the males and is only apparant when a parental effect is present. This parental effect can be observed in the F2 generation even if only the grandfather or the grandmother was treated with LSD. ... The most probable target for LSD is the genetic material present in the germinal cells of the parental generation

Successive crosses with normal animals lead to a dilution of treated genetic material and a proogressive shift toward a normal situation.

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