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Osborne NN, Hiripi L, Neuhoff V. 
“The In Vitro Uptake Of Biogenic Amines By Snail (helix Pomatia) Nervous Tissue”. 
Biochem. Pharmacol.. 1975 Dec. 1;24:2141-2148.
Abstract
The uptake of ['H]5-HT, [3H] dopamine, [3H],noradrenaline and [3H]octopamine into the suboesophageal ganglia of the snail, Helir pomatia, was studied. When tissues were incubated at 25 in mediums containing radioactive amines, t

ISSUES: medium ratios of about ;0: 1, 18: 1, 4: 1 and 5: I for 5-HT, dopamine, noradrenaline and octopamine respectively were obtained after20-30min incubation. Tissues incubated at 25 in mediums containing radioactive amines for 20-30 min showed that 900f the radioactivity was present as unchanged [3H]5-HT, [3H]dopamine, [3H]noradrenaline or a [3H]octopamine. The high t

ISSUES: medium ratios for 5-HT and dopamine. but not for noradrenaline and octopamine, demonstrated saturation kinetics which were dependent upon temperature and sodium ions. From the Lineveaver-Burk plots, two uptake mechanisms for 5-HT at25 were resolved, the high affinity uptake process having a Km1 value of 848 x 10-8 M and Van, value of 0 077 nmole/g per min. while the lower affinity process had a Km2. value of 1.8 x 10- 6 M and a Vmax, value of 066 mole/g per min. At 0 a single uptake mechanism for 5 - HT occurred which gave a Km value of 0 152 x 10- 8 M and a V, value of 0.0203 nmole/g per min. In the case of dopamine, the Lineweaver-Burk plot of 25 showed a single uptake process with values for Km. and Vmax of 1.02 x 10-7M and 0.0673 nmole/g per min respectively. This process did not function at 0. The effects of various agents and ions upon the accumulation processes for snail amines were also studied, and the findings indicate that the same neurons may well accumulate more than one amine type. It is concluded that 5-HT and dopamine uptake in the snail ganglia is a mechanism for inactivating these substances at 25 and that an uptake mechanism for 5-HT also functions at 0. The present results are discussed from the point of view of the monoantines having transmitter functions in the snail CNS.
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