Erowid
 
 
Plants - Drugs Mind - Spirit Freedom - Law Arts - Culture Library  
Erowid References Database
Bockaert J, Premont J, Glowinski J, Thierry AM, Tassin JP. 
“Topographical distribution of dopaminergic innervation and of dopaminergic receptors in the rat striatum. II. Distribution and characteristics of dopamine adenylate cyclase - Interaction of D-LSD with dopaminergetic receptors”. 
Brain Research. 1976;107:303-315.
Abstract
The characteristics of dopamine adenylate cyclase in the rat striatum were first studied on homogenates of fresh tissues. In the assay conditions used, dopamine (10-4 M) stimulated the enzyme activity by 250%. This effect was completely blocked by fluphenazine (10-5 M; Ki = 9 X 10-9 M) and by phentolamine (10-5 M; K =3 x 10-7 M).n-LSD stimulated the adenylatecyclase activity(Km= 1.4 x 10-7 M) by interacting with dopamine receptors; indeed the dopamine effect on the enzyme activity was competitively reduced in presence of D-LSD. L-lsoproterenol (K,, = 10-6 M) activated an adeny]ate cyclase through a receptor distinct from the dopaminergic receptor; this stimulation was not affected by fluphenazine or phentolamine but suppressed by D-propranolol (10-4 M).The topographical distribution of the dopamine, D-LSD and L-isoproterenol adenylate cyclase activities were examined in homogenates prepared from discs punched out on serial frozen (7 C) slices of the striatum. Under this condition, the dopamine maximal stimulation was of 150%. A 4.8-fold progressive decrease in the amount of cyclic AMP produced in presence of dopamine (10-4 M) was observed in the rostrocaudal plane of the structure; the decline of the basal activity was 3.6-fold. The topographical curves of maximal activation of adenylate cyclase by dopamine and D-LSD were superimposable confirming that D-LSD acts on dopaminergic receptors. This topographical distribution of dopamine sensitive adenylate cyclase is comparable on one hand to that of endogenous dopamine and on the other hand to that of the dopamine high affinity uptake activity measured in simultaneous experiments.
Comments and Responses to this Article
#
Submit Comment
[ Cite HTML ]