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Moffat A C. 
“Analytical Chemistry and Drug Metabolism.”. 
Proc.Anal.Div.Chem.Soc.. 1978;15, No.8:237-39.
A short survey of the chemical analysis and metabolism of LSD and cannabinoids is presented. Because LSD is labile, HPLC was found to be superior to GLC for the separation of this drug and its metabolites. A fluorimeter was used as the HEIR detector when determining LSD because of its excellent fluorescence characteristics. The information gained from HPLC-fluorimetry gave the elusion volume and the spectral characteristics. Cannabinoids are not naturally fluorescent but some, e.g. cannabinol (C B N), could be converted into fluorescent compounds when irradiated with W light. This was used in an on-line photo chemical derivatization method for the detection of CON in biological fluids. When a sample was chromatographed the HPLC eluate passed into a photochemical reactor, where it was subjected to a high flux of UV light for a few seconds, which converted it into a fluorescent derivative. The eluate then passed into a fluorimeter with a sensitivity of less than 1 ng. Radioimmuncassays (RIAs) have been reported for both THC and LSD. This technique has the advantage that it can be used on both plasma and urine without extraction and has a high sensitivity. The combination of HPLC with RIA gave the advantages of both techniques. Several ml of urine or plasma were injected on to a reversed phase column (provided that the protein was removed). The HPLC eluate was collected, and a RIA was then used as the HPLC detector. This approach has been developed successfully for the identification and assay of LSD and cannabin oids.
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