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Rappoport M, Cornish H. 
“The Effect of Two Hallucinogens on in Vivo Concentrations of Cyclic AMP in Rat Brain”. 
Toxicol. appl. Pharmacol.. 1974;41(1):172.
The hypothesis that cyclic AMP(cAMP) may play an important role in the function of the central nervous system and may act as a mediator of psychotropic drug effects is based upon a large body of evidence. It has been reported that hallucinogens including d-LSD and dime“hyltryptamine (DMT) cause significant increases in concentrations of cAMP in rat brain stem slices and in vivo as measured following decapitation and rapid dissection and freezing of cerebrum, brain stem, and cerebellum. The presently accepted method of sacrifice of rats prior to cyclic nucleotide assay, i.e., microwave irradiation (1.2 kW) focused on the head of the restrained animal, was used in the present studies. Cyclic AMP was measured either by a modified Giiman assay or by radioimmunoassay. Both methods gave comparable values for cAMP. Following ip administration of 3,4-methylenedioxyamphetamine (MDA) and DMT (0.1 mmol/kg), no significant differences in concentrations of cAMP were found between vehicle control and hallucinogen-injected animals when killed at 2, 5, or 15 min postinjection. After pretreatment with several potent inhibitors of cyclic nucleotide phosphodiesterase there were still no differences in cAMP concentrations between control and hallucinogen-treated rats. Representative cAMP concentrations in Sprague-Dawley rats were as follows (pmol/mg of protein): cerebellum, 21.8; brain stem, 16.5; cortex, 13.7; whole forebrain, 10.5. These basal values are relatively high when compared to brain cAMP concentrations reported in rats killed in a 5-kW microwave oven. Thus, there appears to be an inverse relationship between the rate of enzyme inactivation and post mortem concentrations of cAMP. (Supported by USPHS Grant ES00138.)
Notes # : Abstr. p. 98
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