Erowid References Database
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Drummond AH, Bucher F, Levitan IB.
“d-[3H]Lysergic Acid Diethylamide Binding to Serotonin Receptors in the Molluscan Nervous System”.
J. Biol. Chem.. 1980;255(14):6679-86.
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Abstract
d-[3H]Lyscrgic acid diethylamide (LSD) bound to both dopamine and serotonin (6HT)-sensitive sites in a particulate fraction derived from the central nervous system of the snail Helix pomatia. Conditions were found which enabled the two sites to be studied independently. 13H]LSD appeared to have a slightly higher affinity for dopamine-sensitive binding (K,' = 0.5 nM) than for 5HT-sensitive binding (K,' = 1.2 nM). A pharmacological analysis of the binding indicated that while dopamine and 5HT-related agonists clearly discriminated between the two sites, putative antagonists showed little specificity for dopamine or 5HT-sensitive binding. The pharmacology of 5HT-sensitive [3H]LSD binding was studied in relation to a 5HT-sensitive adenylate cyclase present in a particulate fraction derived from the same tissue. There was a very good correlation between the abilities of a range of agents to act as agonists or antagonists in the 5HT-sensitive adenylate I cyclase assay and their abilities to displace 5HT-sensitive [3H]LSD binding (r = 0.94; p < 0;001). In particular, d-LSD and a number of neuroleptic drugs were inhibitory in both assays in a stereoselective manner. These data suggest that in molluscan tissues, 5HT-sensitive [3H]LSD binding is related to the 5HT receptor which is coupled to adenylate cyclase.
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