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Brunnenberg M, Lindenblatt H, Gouzoulis-Mayfrank E, Kovar KA. 
“Quantitation of N-ethyl-3,4-methylenedioxyamphetamine and its major metabolites in human plasma by high-performance liquid chromatography and fluorescence detection”. 
J Chromatogr B Biomed Sci Appl. 1998 Nov 18;719(1-2):79-85.
A HPLC method has been developed for the analogue of Ecstasy MDE and its major metabolites N-ethyl-4-hydroxy-3-methoxyamphetamine HME and 3,4-methylenedioxyamphetamine MDA in human plasma. In the course of our investigations we found that the methylenedioxyamphetamines and HME exhibit fluorescence at 322 nm. Therefore the detection could be carried out with a fluorescence FL detector. Solid-phase extraction was used for sample preparation and yielded high recovery rates greater than 95. The limit of quantitation for MDE and its metabolites in the extracts was between 1.5 and 8.9 ng/ml and the method standard deviations were less than 5. This sensitive, rapid and reliable analytical method has been used successfully in the quantitation of the substances in plasma samples obtained from 14 volunteers in two clinical studies after p.o. administration of 100 to 140 mg MDE*HCI. The maximum plasma concentrations were 235-465 ng/ml MDE, 67-673 ng/ml HME and 7-33 ng/ml MDA, respectively. Pharmacokinetic parameters have been investigated using the plasma concentration curves.
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